KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
chorismate | enzyme form CM2 | Nicotiana sylvestris | |
1.7 | - |
chorismate | enzyme form CM1, weak positive cooperativity | Nicotiana sylvestris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | enzyme form CM2 | Nicotiana sylvestris | 5829 | - |
plastid | chloroplasts or proplastids, enzyme form CM1 | Nicotiana sylvestris | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
enzyme form CM1, gel filtration | Nicotiana sylvestris |
65000 | - |
enzyme form CM2, gel filtration | Nicotiana sylvestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana sylvestris | - |
2 enzyme forms: CM1 and CM2 | - |
Purification (Comment) | Organism |
---|---|
partial | Nicotiana sylvestris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | the major fraction is formed by enzyme form CM2 | Nicotiana sylvestris | - |
leaf | major fraction is formed by enzyme form CM1 | Nicotiana sylvestris | - |
Storage Stability | Organism |
---|---|
0°C or -20°C, enzyme form CM1, stable for at least 1 month | Nicotiana sylvestris |
0°C or -20°C, enzyme form CM2, stable for at least 2 weeks | Nicotiana sylvestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | - |
Nicotiana sylvestris | Prephenate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
20 min, no denaturation, enzyme form CM1 and CM2 | Nicotiana sylvestris |
52 | - |
20 min, 50% loss of activity, enzyme form CM1 | Nicotiana sylvestris |
52 | - |
5 min, more than 50% loss of activity, enzyme form CM2 | Nicotiana sylvestris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | enzyme form CM1 and CM2 | Nicotiana sylvestris |