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Literature summary for 5.4.99.5 extracted from

  • Zhang, S.; Wilson, D.B.; Ganem, B.
    An engineered chorismate mutase with allosteric regulation (2003), Bioorg. Med. Chem., 11, 3109-3114.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
DELTA102-285 hybrid of chorismate mutase and allosteric domain from P-protein without prephrenate dehydratase Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.226
-
chorismate 37°C, pH 7.8, in presence of P-protein Escherichia coli
0.296
-
chorismate 37°C, pH 7.8 Escherichia coli
0.549
-
prephenate 37°C, pH 7.8, in presence of P-protein Escherichia coli
0.59 1 chorismate 37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine Escherichia coli
0.628
-
chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.5 mM phenylalanine Escherichia coli
1.036
-
chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.05 mM phenylalanine Escherichia coli
2.547
-
chorismate 37°C, pH 7.8, DELTA102-285 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Chorismate Escherichia coli phenylalanine biosynthesis Prephenate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate reversible only in presence of P-protein Escherichia coli Prephenate
-
r
Chorismate phenylalanine biosynthesis Escherichia coli Prephenate
-
r

Subunits

Subunits Comment Organism
dimer SDS-PAGE and gel filtration Escherichia coli

Synonyms

Synonyms Comment Organism
chorismate mutase
-
Escherichia coli
EcCM-R
-
Escherichia coli
P-protein
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
94100
-
prephenate 37°C, pH 7.8, in presence of P-protein Escherichia coli
94140
-
prephenate 37°C, pH 7.8, in presence of P-protein Escherichia coli
140500
-
chorismate 37°C, pH 7.8, in presence of P-protein Escherichia coli
141000
-
chorismate 37°C, pH 7.8, in presence of P-protein Escherichia coli
148900
-
chorismate 37°C, pH 7.8, chorismate mutase Escherichia coli
149000
-
chorismate 37°C, pH 7.8, chorismate mutase Escherichia coli
234000
-
chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.05 mM phenylalanine Escherichia coli
234100
-
chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.05 mM phenylalanine Escherichia coli
253000
-
chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.5 mM phenylalanine Escherichia coli
253400
-
chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.5 mM phenylalanine Escherichia coli
257900
-
chorismate 37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine Escherichia coli
258000
-
chorismate 37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine Escherichia coli
365600
-
chorismate 37°C, pH 7.8, DELTA102-285 Escherichia coli
366000
-
chorismate 37°C, pH 7.8, DELTA102-285 Escherichia coli