Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
DELTA102-285 | hybrid of chorismate mutase and allosteric domain from P-protein without prephrenate dehydratase | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.226 | - |
chorismate | 37°C, pH 7.8, in presence of P-protein | Escherichia coli | |
0.296 | - |
chorismate | 37°C, pH 7.8 | Escherichia coli | |
0.549 | - |
prephenate | 37°C, pH 7.8, in presence of P-protein | Escherichia coli | |
0.59 | 1 | chorismate | 37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine | Escherichia coli | |
0.628 | - |
chorismate | 37°C, pH 7.8, DELTA102-285 in presence of 0.5 mM phenylalanine | Escherichia coli | |
1.036 | - |
chorismate | 37°C, pH 7.8, DELTA102-285 in presence of 0.05 mM phenylalanine | Escherichia coli | |
2.547 | - |
chorismate | 37°C, pH 7.8, DELTA102-285 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | Escherichia coli | phenylalanine biosynthesis | Prephenate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | reversible only in presence of P-protein | Escherichia coli | Prephenate | - |
r | |
Chorismate | phenylalanine biosynthesis | Escherichia coli | Prephenate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | SDS-PAGE and gel filtration | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
chorismate mutase | - |
Escherichia coli |
EcCM-R | - |
Escherichia coli |
P-protein | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
94100 | - |
prephenate | 37°C, pH 7.8, in presence of P-protein | Escherichia coli | |
94140 | - |
prephenate | 37°C, pH 7.8, in presence of P-protein | Escherichia coli | |
140500 | - |
chorismate | 37°C, pH 7.8, in presence of P-protein | Escherichia coli | |
141000 | - |
chorismate | 37°C, pH 7.8, in presence of P-protein | Escherichia coli | |
148900 | - |
chorismate | 37°C, pH 7.8, chorismate mutase | Escherichia coli | |
149000 | - |
chorismate | 37°C, pH 7.8, chorismate mutase | Escherichia coli | |
234000 | - |
chorismate | 37°C, pH 7.8, DELTA102-285 in presence of 0.05 mM phenylalanine | Escherichia coli | |
234100 | - |
chorismate | 37°C, pH 7.8, DELTA102-285 in presence of 0.05 mM phenylalanine | Escherichia coli | |
253000 | - |
chorismate | 37°C, pH 7.8, DELTA102-285 in presence of 0.5 mM phenylalanine | Escherichia coli | |
253400 | - |
chorismate | 37°C, pH 7.8, DELTA102-285 in presence of 0.5 mM phenylalanine | Escherichia coli | |
257900 | - |
chorismate | 37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine | Escherichia coli | |
258000 | - |
chorismate | 37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine | Escherichia coli | |
365600 | - |
chorismate | 37°C, pH 7.8, DELTA102-285 | Escherichia coli | |
366000 | - |
chorismate | 37°C, pH 7.8, DELTA102-285 | Escherichia coli |