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Literature summary for 5.4.99.5 extracted from

  • Woycechowsky, K.J.; Choutko, A.; Vamvaca, K.; Hilvert, D.
    Relative tolerance of an enzymatic molten globule and its thermostable counterpart to point mutation (2008), Biochemistry, 47, 13489-13496.
    View publication on PubMed
Search for more literature for 5.4.99.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli KA13 cells Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
D48G the mutation causes kcat/Km to decrease by 3 fold Methanocaldococcus jannaschii
F77W mutant exhibits decreased activity compared to the wild type enzyme Methanocaldococcus jannaschii
Q88N mutant exhibits decreased activity compared to the wild type enzyme Methanocaldococcus jannaschii
R51Q mutant exhibits decreased activity compared to the wild type enzyme Methanocaldococcus jannaschii
V35A mutant exhibits decreased activity compared to the wild type enzyme Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 chorismate mutant enzyme V35A, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
0.054
-
 chorismate wild type enzyme, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
0.059
-
 chorismate mutant enzyme D48G, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
0.073
-
 chorismate mutant enzyme F77W, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
1.2
-
 chorismate mutant enzyme Q88N, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
1.3
-
 chorismate mutant enzyme R51Q, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography and gel filtration Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate
-
 Methanocaldococcus jannaschii Prephenate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.042
-
 chorismate mutant enzyme Q88N, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
0.41
-
 chorismate mutant enzyme F77W, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
0.5
-
 chorismate mutant enzyme V35A, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
1.3
-
 chorismate mutant enzyme D48G, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
2.6
-
 chorismate mutant enzyme R51Q, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii
3
-
 chorismate wild type enzyme, in PBS buffer (pH 7.5) at 20°C Methanocaldococcus jannaschii