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Literature summary for 5.4.99.63 extracted from

  • Good, N.M.; Martinez-Gomez, N.C.; Beck, D.A.; Lidstrom, M.E.
    Ethylmalonyl coenzyme A mutase operates as a metabolic control point in Methylobacterium extorquens AM1 (2015), J. Bacteriol., 197, 727-735.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.63

Cloned(Commentary)

Cloned (Comment) Organism
gene ecm, quantitative enzyme expression analysis, transcriptional profile of EMC pathway genes during the transition from growth on succinate to growth on ethylamine, overview. Recombinant expression in Escherichia coli Methylorubrum extorquens

Protein Variants

Protein Variants Comment Organism
up growth on ethylamide induces the enzyme, the ethylmalonyl-CoA mutase activity increases to a level sufficient for the observed growth rate at 9 h, which correlates with an upregulation of RNA transcripts for ecm and a decrease in the levels of ethylmalonyl-CoA Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R)-ethylmalonyl-CoA Methylorubrum extorquens
-
 (2S)-methylsuccinyl-CoA
-
 r
(2R)-ethylmalonyl-CoA Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
 (2S)-methylsuccinyl-CoA
-
 r

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens C7C6S9 gene ecm
-
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 C7C6S9 gene ecm
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Methylorubrum extorquens

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:ethylamine-grown cell wild-type Methylobacterium extorquens cells grown at steady state on a limiting concentration of succinate, are then switched to ethylamine as growth substrate, a condition where the cell must make a sudden switch from utilizing the tricarboxylic acid cycle to using the ethylmalonyl-CoA pathway for assimilation Methylorubrum extorquens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R)-ethylmalonyl-CoA
-
 Methylorubrum extorquens (2S)-methylsuccinyl-CoA
-
 r
(2R)-ethylmalonyl-CoA a reversible enzyme Methylorubrum extorquens (2S)-methylsuccinyl-CoA
-
 r
(2R)-ethylmalonyl-CoA
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 (2S)-methylsuccinyl-CoA
-
 r
(2R)-ethylmalonyl-CoA a reversible enzyme Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 (2S)-methylsuccinyl-CoA
-
 r

Synonyms

Synonyms Comment Organism
Ecm
-
 Methylorubrum extorquens
ethylmalonyl coenzyme A mutase
-
 Methylorubrum extorquens
ethylmalonyl-CoA mutase
-
 Methylorubrum extorquens

Cofactor

Cofactor Comment Organism Structure
adenosylcobalamin dependent on Methylorubrum extorquens

General Information

General Information Comment Organism
malfunction overexpression of gene ecm removes control in the ethylmalonyl-CoA pathway Methylorubrum extorquens
metabolism the metabolism of one- and two-carbon compounds by the methylotrophic bacterium Methylobacterium extorquens AM1 involves high carbon flux through the ethylmalonyl-CoA pathway (EMC pathway), metabolic pathway for two carbon metabolism, overview. During growth on ethylamine, the EMC pathway operates as a linear pathway carrying the full assimilatory flux to produce glyoxylate, malate, and succinate, metabolite profiles. Assimilatory carbon enters the ethylmalonyl-CoA pathway directly as acetyl-CoA, bypassing pathways for formaldehyde oxidation/assimilation and the regulatory mechanisms controlling them Methylorubrum extorquens
physiological function the ethylmalonyl-CoA pathway, ethylmalonyl-CoA mutase is a metabolic control point to restrict the production of glyoxylate Methylorubrum extorquens