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Literature summary for 5.5.1.1 extracted from

  • Mazur, P.; Piecken, W.A.; Budihas, S.R.; Williams, S.E.; Wong, S.; Kozarich, J.W.
    cis,cis-Muconate lactonizing enzyme from Trichosporon cutaneum: evidence for a novel class of cycloisomerases in eucaryotes (1994), Biochemistry, 33, 1961-1970.
    View publication on PubMed
Search for more literature for 5.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Cutaneotrichosporon cutaneum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 4 * 43000, SDS-PAGE Cutaneotrichosporon cutaneum
169000
-
 gel filtration Cutaneotrichosporon cutaneum

Organism

Organism UniProt Comment Textmining
Cutaneotrichosporon cutaneum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cutaneotrichosporon cutaneum

Reaction

Reaction Comment Organism Reaction ID
(+)-muconolactone = cis,cis-muconate syn lactonization Cutaneotrichosporon cutaneum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.2
-
-
 Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-Bromo-cis,cis-muconate at 9% of the activity relative to 3-methyl-cis,cis-muconate Cutaneotrichosporon cutaneum ?
-
 ?
3-Chloro-cis,cis-muconate at 11% of the activity relative to 3-methyl-cis,cis-muconate Cutaneotrichosporon cutaneum 5-Chloromuconolactone
-
 ?
3-Fluoro-cis,cis-muconate at 17% of the activity relative to 3-methyl-cis,cis-muconate Cutaneotrichosporon cutaneum (+)-4-Fluoromuconolactone
-
 ?
3-Methyl-cis,cis-muconate
-
 Cutaneotrichosporon cutaneum beta-Methylmuconolactone
-
 ?
cis,cis-Muconate
-
 Cutaneotrichosporon cutaneum (+)-Muconolactone i.e. 5-oxo-2,5-dihydrofuran-2-acetate, , (4S)-muconolactone ?

Subunits

Subunits Comment Organism
tetramer 4 * 43000, SDS-PAGE Cutaneotrichosporon cutaneum