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Literature summary for 5.5.1.1 extracted from

  • Murakami, S.; Takemoto, J.; Takashima, A.; Shinke, R.; Aoki, K.
    Purification and characterization of two muconate cycloisomerase isoenzymes from aniline-assimilating Frateuria species ANA-18 (1998), Biosci. Biotechnol. Biochem., 62, 1129-1133.
    View publication on PubMed
Search for more literature for 5.5.1.1

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 1.0 mM, complete inhibition of MC I and MC II Frateuria sp.
EDTA 1.0 mM, complete inhibition of MC I and MC II Frateuria sp.
HgCl2 0.5 mM, complete inhibition of enzyme form MC I and MC II Frateuria sp.
PCMB
-
 Frateuria sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 6% of the activity with Mn2+, enzyme form MC II Frateuria sp.
Fe2+ 0.4% of the activity with Mn2+, enzyme form MC II Frateuria sp.
Mg2+ 28% of the activity with Mn2+, enzyme form MC I. 17% of the activity with Mn2+, enzyme form MC II Frateuria sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000, SDS-PAGE Frateuria sp.
400000
-
 gel filtration Frateuria sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis,cis-muconate Frateuria sp. different inducible production of MC I and MC II suggest that two catechol branches involved in the beta-ketoadipate pathway function in Frateuria sp. ANA-18 muconolactone
-
 ?
cis,cis-muconate Frateuria sp. ANA-18 different inducible production of MC I and MC II suggest that two catechol branches involved in the beta-ketoadipate pathway function in Frateuria sp. ANA-18 muconolactone
-
 ?

Organism

Organism UniProt Comment Textmining
Frateuria sp.
-
 ANA-18
-
Frateuria sp. ANA-18
-
 ANA-18
-

Purification (Commentary)

Purification (Comment) Organism
-
 Frateuria sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Frateuria sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis,cis-muconate
-
 Frateuria sp. muconolactone
-
 ?
cis,cis-muconate different inducible production of MC I and MC II suggest that two catechol branches involved in the beta-ketoadipate pathway function in Frateuria sp. ANA-18 Frateuria sp. muconolactone
-
 ?
cis,cis-muconate
-
 Frateuria sp. ANA-18 muconolactone
-
 ?
cis,cis-muconate different inducible production of MC I and MC II suggest that two catechol branches involved in the beta-ketoadipate pathway function in Frateuria sp. ANA-18 Frateuria sp. ANA-18 muconolactone
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Frateuria sp.

Synonyms

Synonyms Comment Organism
MC II
-
 Frateuria sp.
MC O
-
 Frateuria sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 10 min, 65% loss of activity of enzyme form MC I Frateuria sp.
75
-
 10 min, 19% loss of activity of enzyme form MC II Frateuria sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 enzyme form MC I and MC II Frateuria sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 enzyme form MCI shows more than 80% of maximal activity in the range pH 5.5-7.5 Frateuria sp.
6 7 enzyme form MCII shows more than 80% of maximal activity in the range pH 6.0-7.0 Frateuria sp.