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Literature summary for 5.5.1.1 extracted from

  • Blasco, R.; Ramos, J.L.; Wittich, R.M.
    Pseudomonas aeruginosa strain RW41 mineralizes 4-chlorobenzenesulfonate, the major polar by-product from DDT manufacturing (2008), Environ. Microbiol., 10, 1591-1600.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme induction by 4-chlorobenzenesulfonic acid Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis,cis-muconate Pseudomonas aeruginosa i.e. cis,cis-hexadienedioate or (2Z,4Z)-hexa-2,4-dienedioate, in vitro activity muconolactone i.e. (2,5-dihydro-5-oxofuran-2-yl)acetate ?
cis,cis-muconate Pseudomonas aeruginosa RW41 i.e. cis,cis-hexadienedioate or (2Z,4Z)-hexa-2,4-dienedioate, in vitro activity muconolactone i.e. (2,5-dihydro-5-oxofuran-2-yl)acetate ?
additional information Pseudomonas aeruginosa the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview ?
-
?
additional information Pseudomonas aeruginosa RW41 the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa RW41
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by anion exchange chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.017
-
substrate 3-chloro-cis,cis-muconate, crude enzyme extrat Pseudomonas aeruginosa
0.035
-
substrate cis,cis-muconate, crude enzyme extract Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chloro-cis,cis-muconate
-
Pseudomonas aeruginosa 4-chloro-muconolactone unstable ?
3-chloro-cis,cis-muconate
-
Pseudomonas aeruginosa RW41 4-chloro-muconolactone unstable ?
3-chloro-cis,cis-muconate in vitro activity Pseudomonas aeruginosa cis-dienelactone + protoanemonin + Cl- + H2O + CO2 product ratio is 76:24, protoanemonin is antibiotically active ?
3-chloro-cis,cis-muconate in vitro activity Pseudomonas aeruginosa RW41 cis-dienelactone + protoanemonin + Cl- + H2O + CO2 product ratio is 76:24, protoanemonin is antibiotically active ?
cis,cis-muconate
-
Pseudomonas aeruginosa muconolactone
-
?
cis,cis-muconate i.e. cis,cis-hexadienedioate or (2Z,4Z)-hexa-2,4-dienedioate, in vitro activity Pseudomonas aeruginosa muconolactone i.e. (2,5-dihydro-5-oxofuran-2-yl)acetate ?
cis,cis-muconate
-
Pseudomonas aeruginosa RW41 muconolactone
-
?
cis,cis-muconate i.e. cis,cis-hexadienedioate or (2Z,4Z)-hexa-2,4-dienedioate, in vitro activity Pseudomonas aeruginosa RW41 muconolactone i.e. (2,5-dihydro-5-oxofuran-2-yl)acetate ?
additional information the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview Pseudomonas aeruginosa ?
-
?
additional information the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview Pseudomonas aeruginosa RW41 ?
-
?

Synonyms

Synonyms Comment Organism
(chloro)-muconate cycloisomerase
-
Pseudomonas aeruginosa
MCI
-
Pseudomonas aeruginosa