Literature summary for 5.5.1.13 extracted from

Kawaide, H.; Sassa, T.; Kamiya, Y.
Functional analysis of the two interacting cyclase domains in ent-kaurene synthase from the fungus Phaeosphaeria sp. L487 and a comparison with cyclases from higher plants (2000), J. Biol. Chem., 275, 2276-2280.

Search for more literature for 5.5.1.13

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli of a recombinant GST fused enzyme	Cucurbita maxima
expression in Escherichia coli of a recombinant GST fused enzyme	Phaeosphaeria sp.

Protein Variants

Protein Variants	Comment	Organism
D320A	the mutation leads to complete enzyme activity	Phaeosphaeria sp.
D656A	the mutation causes a small reduction in activity	Phaeosphaeria sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	inhibits activity in cell free extract containing the enzyme	Phaeosphaeria sp.
additional information	substrate inhibition	Cucurbita maxima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00023	-	copalyl diphosphate	at pH 8	Phaeosphaeria sp.
0.00873	-	geranylgeranyl diphosphate	at pH 8	Phaeosphaeria sp.
0.0169	-	geranylgeranyl diphosphate	at pH 8	Cucurbita maxima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
106000	-	calculated from amino acid sequence	Phaeosphaeria sp.

Organism

Organism	UniProt	Comment	Textmining
Cucurbita maxima	-	pumpkin	-
Phaeosphaeria sp.	O13284	from mycelia	-

Purification (Commentary)

Purification (Comment)	Organism
cell free extract	Phaeosphaeria sp.
recombinant enzyme, using Sepharose 4B glutathione affinity resin and SDS PAGE electrophoresis	Phaeosphaeria sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00075	-	at pH 8	Phaeosphaeria sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
copalyl diphosphate	bifunctional enzyme	Phaeosphaeria sp.	ent-kaurene + diphosphate	-	?
geranylgeranyl diphosphate	-	Cucurbita maxima	copalyl diphosphate	-	?
geranylgeranyl diphosphate	-	Phaeosphaeria sp.	copalyl diphosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00117	-	copalyl diphosphate	at pH 8	Phaeosphaeria sp.
0.0308	-	geranylgeranyl diphosphate	at pH 8	Phaeosphaeria sp.
0.035	-	geranylgeranyl diphosphate	at pH 8	Cucurbita maxima

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Release 2023.1 (January 2023)