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Literature summary for 5.6.2.1 extracted from
- Purification and properties of type 1 topoisomerase from chicken erythrocytes: mechanism of eukaryotic topoisomerase action (1982), Biochemistry, 21, 1155-1161.
General Stability
| General Stability | Organism |
|---|---|
| VO3- enhances thermostability | Gallus gallus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Gallus gallus | 5634 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
sucrose density gradient centrifugation | Gallus gallus |
| 65000 | - |
glycerol density gradient centrifugation | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP-independent breakage of single-stranded DNA, followed by passage and rejoining | enzyme closes its substrate DNA molecule after the removal of each superhelical turn, dissociation of the enzyme substrate complex may but does not necessarily occur after each cycle of the reaction | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Gallus gallus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, without stabilizer, half-life: 1 week. 0.1 mM NaVO3, increases storage life approximately 3fold | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| supercoiled DNA | relaxation | Gallus gallus | relaxed closed circular DNA | - |
? |  |
| supercoiled DNA | pBR322 DNA | Gallus gallus | relaxed closed circular DNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 62000, SDS-PAGE | Gallus gallus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
half-life in absence of VO3-: 5 min, half-life in presence of 0.1 mM VO3-: 60 min | Gallus gallus |
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