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Literature summary for 5.6.2.1 extracted from

  • Riou, G.F.; Gabillot, M.; Douc-Rasy, S.; Kayser, A.; Barrois, M.
    A type I DNA topoisomerase from Trypanosoma cruzi (1983), Eur. J. Biochem., 134, 479-484.
    View publication on PubMed
Search for more literature for 5.6.2.1

Activating Compound

Activating Compound Comment Organism Structure
spermidine enhances relaxation activity Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
Berenil
-
 Trypanosoma cruzi
Ethidium bromide
-
 Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Trypanosoma cruzi 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enhances relaxation activity Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 1 * 65000, SDS-PAGE Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trypanosoma cruzi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
supercoiled DNA relaxation Trypanosoma cruzi relaxed closed circular DNA
-
 ?
supercoiled DNA positive and negative supercoiled DNA Trypanosoma cruzi relaxed closed circular DNA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37
-
 Trypanosoma cruzi

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 active in this range Trypanosoma cruzi

pH Range

pH Minimum pH Maximum Comment Organism
5.4 8.5 active in this range Trypanosoma cruzi