Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ethidium bromide | - |
Alcaligenes sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Mg2+ or Ca2+ required | Alcaligenes sp. | |
Mg2+ | optimal concentration is about 5 mM | Alcaligenes sp. | |
Mg2+ | Mg2+ or Ca2+ required | Alcaligenes sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Alcaligenes sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
supercoiled DNA | removes superhelical turns from a negatively twisted DNA, no effect on positive superhelical turns | Alcaligenes sp. | relaxed closed circular DNA | - |
? | |
supercoiled DNA | relaxation | Alcaligenes sp. | relaxed closed circular DNA | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
pH 7.5, 10 min, stable | Alcaligenes sp. |
60 | - |
pH 7.5, 10 min, complete inactivation | Alcaligenes sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10 | - |
Alcaligenes sp. |