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Literature summary for 5.6.2.1 extracted from
- Isolation and partial characterization of two distinct DNA topoisomerases from cauliflower inflorescence (1983), J. Biochem., 91, 1337-1342.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| spermidine | 2 mM, stimulates | Brassica oleracea |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Brassica oleracea | |
| Nalidixic acid | no inhibition | Brassica oleracea | |
| NEM | - |
Brassica oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Brassica oleracea | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10-15 mM stimulates | Brassica oleracea | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brassica oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| inflorescence | - |
Brassica oleracea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| supercoiled DNA | relaxation | Brassica oleracea | relaxed closed circular DNA | - |
? | |
| supercoiled DNA | pBR322 DNA | Brassica oleracea | relaxed closed circular DNA | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
30 min, inactivation | Brassica oleracea |
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