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Literature summary for 5.6.2.1 extracted from
- Purification and characterization of a DNA topoisomerase I from Bifidobacterium breve (1989), Agric. Biol. Chem., 53, 3357-3358.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| actinomycin D | - |
Bifidobacterium breve |  |
| camptothecins | no inhibition | Bifidobacterium breve | |
| Ethidium bromide | - |
Bifidobacterium breve | |
| Na+ | above 150 mM | Bifidobacterium breve | |
| Neomycin sulfate | - |
Bifidobacterium breve | |
| single-stranded DNA | heat-denatured single-stranded DNA | Bifidobacterium breve | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | divalent cation required Ca2+ or Mg2+ | Bifidobacterium breve | |
| K+ | stimulates | Bifidobacterium breve | |
| Mg2+ | Mg2+ or Ca2+ required | Bifidobacterium breve | |
| Mg2+ | optimal concentration 5-15 mM | Bifidobacterium breve | |
| Mn2+ | stimulates | Bifidobacterium breve | |
| Na+ | stimulates, optimal concentration: 50-100 mM | Bifidobacterium breve | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium breve | - |
- |
- |
| Bifidobacterium breve YIT4006 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bifidobacterium breve |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for more than 1 year | Bifidobacterium breve |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| supercoiled DNA | relaxation | Bifidobacterium breve | relaxed closed circular DNA | - |
? | |
| supercoiled DNA | specific for negatively supercoiled DNA, no activity with positively supercoiled DNA | Bifidobacterium breve | relaxed closed circular DNA | - |
? | |
| supercoiled DNA | relaxation | Bifidobacterium breve YIT4006 | relaxed closed circular DNA | - |
? | |
| supercoiled DNA | specific for negatively supercoiled DNA, no activity with positively supercoiled DNA | Bifidobacterium breve YIT4006 | relaxed closed circular DNA | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Bifidobacterium breve |
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Release 2023.1 (January 2023)
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