Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Nicotiana tabacum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | CaCl2 significantly enhances activity | Nicotiana tabacum | |
Mg2+ | MgCl2 significantly enhances activity | Nicotiana tabacum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE, may exist as a multimeric protein in the native state | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
var. xanthi | - |
Purification (Comment) | Organism |
---|---|
- |
Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Nicotiana tabacum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Nicotiana tabacum |
Storage Stability | Organism |
---|---|
-70°C, 50 mM Tris-HCl, pH 7.8, 10 mM 2-mercaptoethanol, 0.5 mM dithiothreitol, 0.5 mM PMSF, 15% glycerol, stable for at least 6 months | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
supercoiled DNA | relaxation | Nicotiana tabacum | relaxed closed circular DNA | - |
? | |
supercoiled DNA | positive and negative supercoiled DNA | Nicotiana tabacum | relaxed closed circular DNA | - |
? | |
supercoiled DNA | negatively supercoiled plasmid DNA | Nicotiana tabacum | relaxed closed circular DNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE, may exist as a multimeric protein in the native state | Nicotiana tabacum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | - |
Nicotiana tabacum |