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Literature summary for 5.6.2.1 extracted from

  • Heath-Pagliuso, S.; Cole, A.D.; Kmiec, E.B.
    Purification and characterization of a type-I topoisomerase from cultured tobacco cells (1990), Plant Physiol., 94, 599-606.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Nicotiana tabacum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ CaCl2 significantly enhances activity Nicotiana tabacum
Mg2+ MgCl2 significantly enhances activity Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, SDS-PAGE, may exist as a multimeric protein in the native state Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
var. xanthi
-

Purification (Commentary)

Purification (Comment) Organism
-
Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Nicotiana tabacum

Storage Stability

Storage Stability Organism
-70°C, 50 mM Tris-HCl, pH 7.8, 10 mM 2-mercaptoethanol, 0.5 mM dithiothreitol, 0.5 mM PMSF, 15% glycerol, stable for at least 6 months Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
supercoiled DNA relaxation Nicotiana tabacum relaxed closed circular DNA
-
?
supercoiled DNA positive and negative supercoiled DNA Nicotiana tabacum relaxed closed circular DNA
-
?
supercoiled DNA negatively supercoiled plasmid DNA Nicotiana tabacum relaxed closed circular DNA
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE, may exist as a multimeric protein in the native state Nicotiana tabacum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37
-
Nicotiana tabacum