Inhibitors | Comment | Organism | Structure |
---|---|---|---|
actinomycin D | 10 mg/l, complete inhibition | Rhodobacter capsulatus | |
ATP | 5 mM, 75% inhibition, ccomplete inhibition at 10 mM, regardless of Mg2+ concentration | Rhodobacter capsulatus | |
Ethidium bromide | 0.25 mg/l, complete inhibition | Rhodobacter capsulatus | |
heparin | 1 mg/ml, complete inhibition | Rhodobacter capsulatus | |
Hoechst 33258 | 5 mg/l, 70% inhibition | Rhodobacter capsulatus | |
K+ | above 150 mM | Rhodobacter capsulatus | |
M13 DNA | strong inhibitor of relaxing activity | Rhodobacter capsulatus | |
Na+ | above 150 mM | Rhodobacter capsulatus | |
NEM | 2 mM, complete inhibition | Rhodobacter capsulatus | |
spermidine | 4 mM, complete inhibition | Rhodobacter capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | optimal concentration is 4-8 mM, can replace the effect of Mg2+ | Rhodobacter capsulatus | |
K+ | stimulates below 60 mM | Rhodobacter capsulatus | |
Mg2+ | 2-8 mM, absolute requirement for divalent cation | Rhodobacter capsulatus | |
Mn2+ | optimal concentration is 2-8 mM, can replace the effect of Mg2+ | Rhodobacter capsulatus | |
Na+ | stimulates below 60 mM | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodobacter capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
negatively supercoiled PUC18 DNA | unable to relax positively supercoiled DNA | Rhodobacter capsulatus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Rhodobacter capsulatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
180 min, stable | Rhodobacter capsulatus |
25 | - |
180 min, about 80% loss of activity. 60 min, about 50% loss of activity | Rhodobacter capsulatus |
37 | - |
180 min, complete loss of activity. 60 min, about 65% loss of activity | Rhodobacter capsulatus |
45 | - |
60 min, 90% loss of activity | Rhodobacter capsulatus |
55 | - |
15 min, complete inactivation | Rhodobacter capsulatus |