Any feedback?
Literature summary for 5.6.2.1 extracted from
- Alteration of Escherichia coli topoisomerase IV conformation upon enzyme binding to positively supercoiled DNA (2006), J. Biol. Chem., 281, 18927-18932.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
E monomer | Escherichia coli |
| 84000 | - |
C monomer | Escherichia coli |
| 140000 | - |
EE dimer | Escherichia coli |
| 154000 | - |
CE dimer | Escherichia coli |
| 168000 | - |
CC dimer | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | silver staining, Western blotting, cross-linking of topo IV with glutaraldehyde in the absence of DNA. Southern blotting, cross-linking of Topo IV in the presence of DNA, composed of two molecules each of ParC and ParE, topo IV binds the gate segment and the transport segment of DNA. The Par E subunit dimerize prior to strand passage | Escherichia coli |
| More | other cross-linking reagents than glutaraldehyde tested. Western blot analysis of cross-linked topo IV bound to DNA. Model for topo IV tetramers bound to (-)- and (+)-SC DNA shown | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| topoisomerase IV | - |
Escherichia coli |
| topoIV | - |
Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
