Cloned (Comment) | Organism |
---|---|
BPV1 glutathione S-transferase (GST)-E1, BPV1 GST-E1 DBD, BPV1 E2, and the human papillomavirus type 11 (HPV11) GST-E1 truncation mutant (aa 589 to 649) expressed in Escherichia coli BL21(DE3) | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Human Topo I purified from extracts of baculovirus-infected Sf9 or Hi-5 cells | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining | demonstrated that human Topo I specifically stimulates the origin binding activity of PV E1, apparently through a protein-protein interaction. Topo I stimulates the origin binding activity of papillomavirus protein E1. Topo I stimulation of E1 binding is highly specific, verified by enzyme-linked immunosorbent assays using a nonorigin DNA fragment, Topo I stimulation of E1 DNA binding activity is origin specific. E1 does not affect the DNA binding activity of Topo I. DNA modulates the interaction between E1 and Topo I (ELISA-based protein interaction assays). Topo I and E2 stimulate the origin binding of E1 independently(enzyme-linked immunosorbent assay) | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Topo I | - |
Homo sapiens |
Topoisomerase I | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
At 37°C, E1 double hexamers are preferentially formed. The addition of ATP/Mg2+ stimulated the binding activity of E1 at 37°C, but not at 25°C. Optimal stimulation of binding of E1 by Topo I to origin DNA occurred at 37°C in the presence of ATP/Mg 2+ | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | The effects of ATP/Mg2+ and temperature on the ability of E1 to bind origin DNA in the presence and absence of Topo I is shown. Increasing amounts of E1 were incubated with radiolabeled PV origin DNA at 37°C or 25°C in the presence or absence of ATP/Mg2+ | Homo sapiens |