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Literature summary for 5.6.2.1 extracted from

  • Pham, M.H.; Kondapalli, N.; Reckord, C.L.; Foglesong, P.D.
    Interleukin-2 induces the activities of DNA topoisomerase I and DNA topoisomerase II in HuT 78 cells (2009), Biochem. Biophys. Res. Commun., 390, 577-580.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.1

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus soluble Homo sapiens 5634
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens human cells can rapidly activate and deactivate DNA topoisomerase I in response to a signal molecule, the activity of DNA topoisomerase I is highly regulated in HuT 78 cells upon treatment with interleukin-2, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from Hut 78 cells by nuclear extract preparation Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HUT-78 cell a human cutaneous T cell lymphoma cell line Homo sapiens
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T-cell
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 Homo sapiens
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 quantitative detection of enzyme activity in Hut 78 cells over several hours after transient induction by interleukin-2, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information human cells can rapidly activate and deactivate DNA topoisomerase I in response to a signal molecule, the activity of DNA topoisomerase I is highly regulated in HuT 78 cells upon treatment with interleukin-2, overview Homo sapiens ?
-
 ?
additional information substrate is pBR322 or pHC624 supercoiled DNA Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
DNA topoisomerase I
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 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens the enzyme expression is transiently induced up to 11fold by interleukin-2 at 1000 U/ml after 10 h up