Literature summary for 5.6.2.1 extracted from

Lebedeva, N.A.; Rechkunova, N.I.; Agama, K.; Pommier, Y.; Lavrik, O.I.
Interaction of DNA topoisomerase 1 with DNA intermediates and proteins of base excision repair (2009), Biochemistry (Moscow), 74, 1278-1284.

Search for more literature for 5.6.2.1

Protein Variants

Protein Variants	Comment	Organism
Y723F	the active site mutant shows abolished formation of Top1 covalent adducts with DNA structures	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	partial inhibition of the Top1-DNAadduct formation upon addition of poly(ADP ribose) polymerase 1 in the absence of NAD+ is shown, whereas in the presence of NAD+ formation of a high molecular weight product, most likely corresponding to poly(ADP) ribosylated Top1-DNA adduct, is observed	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	-	Homo sapiens	5634	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	Top1 relaxes supercoiled structures in DNA, thus creating a nick. Top1 cleaves a phosphodiester bond near the 3' end of the nick and forms a covalent bond with the 3' end of the newly formed break. This process involves the catalytic tyrosine residue necessary for nucleophilic attack and disruption of a phosphodiester bond in DNA. and it involves poly(ADP-ribose) polymerase 1, that phosphoribosylates the enzyme and affect binding to DNA, overview. Interaction of human recombinant DNA topoisomerase 1 with linear and circular DNA structures containing a nick or short gap but lacking a specific Top1 recognition site, using circular M13mp19 phage DNA and the 34mer DNA duplex containing uracil in position 16 of one of the duplex strands (U-G) as substrate. Partial inhibition of the Top1-DNAadduct formation upon addition of poly(ADP ribose) polymerase 1 in the absence of NAD+ is shown, whereas in the presence of NAD+ formation of a high molecular weight product, most likely corresponding to poly(ADP) ribosylated Top1-DNA adduct, is observed. Top1 covalent adducts with DNA containing a singlestranded break or a short gap in the absence of a recognition site are also formed with involvement of the catalytic tyrosine residue in the enzyme active center	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
DNA topoisomerase 1	-	Homo sapiens
Top1	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.6	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)