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Literature summary for 5.6.2.1 extracted from

  • Xu, X.; Leng, F.
    A rapid procedure to purify Escherichia coli DNA topoisomerase I (2011), Protein Expr. Purif., 77, 214-219.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain RLM1180/pTI-1 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Escherichia coli
0.00000752
-
 negatively supercoiled plasmid pXXZ06 DNA pH 7.9, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme by polymin P and ammonium sulfate fractionations, cation- and anion-exchange chromatography, purified DNA topoisomerase I is free of DNase contamination, method development, overview Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31700
-
 purified enzyme, pH 7.9, 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
negatively supercoiled plasmid pXXZ06 DNA
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
DNA topoisomerase I
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 negatively supercoiled plasmid pXXZ06 DNA pH 7.9, 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 assay at Escherichia coli