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Literature summary for 6.1.1.10 extracted from

  • Burnell, J.N.; Whatley, F.R.
    Sulphur metabolism in Paracoccus denitrificans. Purification, properties and regulation of cysteinyl- and methionyl-tRNA synthetase (1977), Biochim. Biophys. Acta, 481, 266-278.
    View publication on PubMed
Search for more literature for 6.1.1.10

Activating Compound

Activating Compound Comment Organism Structure
cysteic acid stimulates Paracoccus denitrificans
cysteine stimulates Paracoccus denitrificans
SO32- stimulates Paracoccus denitrificans

General Stability

General Stability Organism
stability is increased by presence of a reducing agent. In the presence of 5 mM DTT purified enzyme is stable for at least 8 months at -15°C Paracoccus denitrificans

Inhibitors

Inhibitors Comment Organism Structure
3'-AMP
-
 Paracoccus denitrificans
5'-AMP
-
 Paracoccus denitrificans
ADP
-
 Paracoccus denitrificans
ATP in free form Paracoccus denitrificans
D-methionine
-
 Paracoccus denitrificans
EDTA 10 mM, weak Paracoccus denitrificans
glutathione
-
 Paracoccus denitrificans
iodoacetamide 10 mM, weak Paracoccus denitrificans
Mg2+ in free form Paracoccus denitrificans
NaF 10 mM, weak Paracoccus denitrificans
NEM
-
 Paracoccus denitrificans
O-acetylserine
-
 Paracoccus denitrificans
p-chloromercuribenzoate
-
 Paracoccus denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
 L-Met
-
 Paracoccus denitrificans
0.31
-
 selenomethionine
-
 Paracoccus denitrificans
0.383
-
 norleucine
-
 Paracoccus denitrificans
0.87
-
 ATP
-
 Paracoccus denitrificans
28.6
-
 norleucine
-
 Paracoccus denitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ supports methionine-dependent ATP-diphosphate exchange with 14% of the efficiency of Mg2+ Paracoccus denitrificans
Co2+ supports methionine-dependent ATP-diphosphate exchange with 20% of the efficiency of Mg2+ Paracoccus denitrificans
Mg2+ absolutely dependent on Paracoccus denitrificans
Mg2+ 2 Paracoccus denitrificans
Mn2+ supports methionine-dependent ATP-diphosphate exchange with 15% of the efficiency of Mg2+ Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
-
-
Paracoccus denitrificans 8944
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paracoccus denitrificans

Storage Stability

Storage Stability Organism
-15°C, 5 mM DTT, purified enzyme stable for at least 8 months Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
 Paracoccus denitrificans AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
ATP + L-methionine + tRNAMet
-
 Paracoccus denitrificans 8944 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
ATP + L-norleucine + tRNAMet
-
 Paracoccus denitrificans AMP + diphosphate + L-norleucyl-tRNAMet
-
 ?
ATP + methionine + hydroxylamine
-
 Paracoccus denitrificans methionine hydroxamate + AMP + diphosphate
-
 ?
ATP + methionine + hydroxylamine
-
 Paracoccus denitrificans 8944 methionine hydroxamate + AMP + diphosphate
-
 ?
ATP + selenomethionine + tRNAMet
-
 Paracoccus denitrificans AMP + diphosphate + selenomethionyl-tRNAMet
-
 ?
ATP + selenomethionine + tRNAMet
-
 Paracoccus denitrificans 8944 AMP + diphosphate + selenomethionyl-tRNAMet
-
 ?
additional information methionine-dependent ATP-diphosphate exchange Paracoccus denitrificans ?
-
 ?
additional information also catalyzes L-homocysteine, L-selenocysteine and norleucine-dependent ATP-diphosphate exchange Paracoccus denitrificans ?
-
 ?
additional information methionine-dependent ATP-diphosphate exchange Paracoccus denitrificans 8944 ?
-
 ?
additional information also catalyzes L-homocysteine, L-selenocysteine and norleucine-dependent ATP-diphosphate exchange Paracoccus denitrificans 8944 ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Paracoccus denitrificans
8 8.5
-
 Paracoccus denitrificans