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Literature summary for 6.1.1.10 extracted from
- Cloning and characterization of mitochondrial methionyl-tRNA synthetase from a pathogenic fungi Candida albicans (1998), Gene, 215, 311-318.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli BL21(DE3), functional complementation of an enzyme-deficient Escherichia coli strain | Candida albicans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | mitochondrial isozyme | Candida albicans | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | Candida albicans | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | O74634 | class I enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | - |
Candida albicans | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
| ATP + L-methionine + tRNAMet | also utilizes both subtypes of Escherichia coli tRNAMet | Candida albicans | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Methionine translase | - |
Candida albicans |
| Methionine--tRNA ligase | - |
Candida albicans |
| Methionyl tRNA synthetase | - |
Candida albicans |
| Methionyl-transfer ribonucleate synthetase | - |
Candida albicans |
| Methionyl-transfer ribonucleic acid synthetase | - |
Candida albicans |
| Methionyl-transfer RNA synthetase | - |
Candida albicans |
| MetRS | - |
Candida albicans |
| Synthetase, methionyl-transfer ribonucleate | - |
Candida albicans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Candida albicans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Candida albicans | |
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