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Literature summary for 6.1.1.11 extracted from

  • Landeka, I.; Filipic-Rocak, S.; Zinic, B.; Weygand-Durasevic, I.
    Characterization of yeast seryl-tRNA synthetase active site mutants with improved discrimination against substrate analogues (2000), Biochim. Biophys. Acta, 1480, 160-170.
    View publication on PubMed
Search for more literature for 6.1.1.11

Activating Compound

Activating Compound Comment Organism Structure
tRNASer promotes the binding of the other substrates, probably due to a conformational change in motif 2 region Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutants in Escherichia coli BL21(DE3) Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
A289V mutations in the active site mutant SerRS11, mutant is less sensitive to inhibition by serine hydroxamate and 5'-O-[N-(L-seryl)-sulfamoyl]adenosine Saccharomyces cerevisiae
D288Y mutations in the active site mutant SerRS11, mutant is less sensitive to inhibition by serine hydroxamate and 5'-O-[N-(L-seryl)-sulfamoyl]adenosine Saccharomyces cerevisiae
K287R mutations in the active site mutant SerRS12, mutant is less sensitive to inhibition by serine hydroxamate and 5'-O-[N-(L-seryl)-sulfamoyl]adenosine Saccharomyces cerevisiae
K287T mutations in the active site mutant SerRS11, mutant is less sensitive to inhibition by serine hydroxamate and 5'-O-[N-(L-seryl)-sulfamoyl]adenosine Saccharomyces cerevisiae
additional information construction of the active site mutants SerRS11 and SerRS12 Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
5'-O-[N-(L-Seryl)-sulfamoyl]adenosine stable analogue of aminoacyl adenylate intermediate Saccharomyces cerevisiae
serine hydroxamate serine analogue Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics for the ATP-diphosphate exchange reaction Saccharomyces cerevisiae
0.00004
-
 tRNASer wild-type enzyme, mutant SerRS11, and mutant SerRS12, pH 7.2, 30°C Saccharomyces cerevisiae
0.011
-
 ATP wild-type enzyme, pH 7.2, 30°C Saccharomyces cerevisiae
0.018
-
 L-serine wild-type enzyme, pH 7.2, 30°C Saccharomyces cerevisiae
0.08
-
 ATP mutant SerRS11, pH 7.2, 30°C Saccharomyces cerevisiae
0.12
-
 ATP mutant SerRS12, pH 7.2, 30°C Saccharomyces cerevisiae
0.14
-
 L-serine mutant SerRS12, pH 7.2, 30°C Saccharomyces cerevisiae
0.2
-
 L-serine mutant SerRS11, pH 7.2, 30°C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-serine + tRNASer Saccharomyces cerevisiae
-
 AMP + diphosphate + L-seryl-tRNASer
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutants from Escherichia coli Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
ATP + L-serine + tRNASer = AMP + diphosphate + L-seryl-tRNASer catalytic mechanism, specificity of substrate binding is mediated by the amino acids in motif 2 loop Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASer
-
 Saccharomyces cerevisiae AMP + diphosphate + L-seryl-tRNASer
-
 ?
ATP + L-serine + tRNASer amino acids within motif 2 loop are involved in specific binding of L-serine and ATP to the enzyme Saccharomyces cerevisiae AMP + diphosphate + L-seryl-tRNASer
-
 ?
additional information the enzyme also performs the ATP-diphosphate exchange reaction Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
62 kDa RNA-binding protein
-
 Saccharomyces cerevisiae
Serine transfer RNA synthetase
-
 Saccharomyces cerevisiae
Serine translase
-
 Saccharomyces cerevisiae
Serine--tRNA ligase
-
 Saccharomyces cerevisiae
SerRS
-
 Saccharomyces cerevisiae
SerRSmt
-
 Saccharomyces cerevisiae
SERSEC
-
 Saccharomyces cerevisiae
Seryl-transfer ribonucleate synthetase
-
 Saccharomyces cerevisiae
Seryl-transfer ribonucleic acid synthetase
-
 Saccharomyces cerevisiae
Seryl-transfer RNA synthetase
-
 Saccharomyces cerevisiae
Seryl-tRNA synthetase
-
 Saccharomyces cerevisiae
Synthetase, seryl-transfer ribonucleate
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
 ATP wild-type enzyme, pH 7.2, 30°C Saccharomyces cerevisiae
0.22
-
 L-serine wild-type enzyme, pH 7.2, 30°C Saccharomyces cerevisiae
0.28
-
 tRNASer wild-type enzyme, pH 7.2, 30°C Saccharomyces cerevisiae
0.4
-
 tRNASer mutant SerRS11, pH 7.2, 30°C Saccharomyces cerevisiae
0.46
-
 tRNASer mutant SerRS12, pH 7.2, 30°C Saccharomyces cerevisiae
0.59
-
 ATP mutant SerRS11, pH 7.2, 30°C Saccharomyces cerevisiae
0.78
-
 L-serine mutant SerRS12, pH 7.2, 30°C Saccharomyces cerevisiae
1.1
-
 L-serine mutant SerRS11, pH 7.2, 30°C Saccharomyces cerevisiae
1.16
-
 ATP mutant SerRS12, pH 7.2, 30°C Saccharomyces cerevisiae
6.08
-
 ATP mutant SerRS11, pH 7.2, 30°C Saccharomyces cerevisiae
6.08
-
 ATP mutant SerRS12, pH 7.2, 30°C Saccharomyces cerevisiae
6.08
-
 L-serine mutant SerRS12, pH 7.2, 30°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.21
-
 5'-O-[N-(L-Seryl)-sulfamoyl]adenosine recombinant wild-type enzyme, pH 7.2, 30°C Saccharomyces cerevisiae
0.28
-
 5'-O-[N-(L-Seryl)-sulfamoyl]adenosine recombinant mutant SerRS11, pH 7.2, 30°C Saccharomyces cerevisiae
2.2
-
 serine hydroxamate recombinant wild-type enzyme, pH 7.2, 30°C Saccharomyces cerevisiae
3.1
-
 5'-O-[N-(L-Seryl)-sulfamoyl]adenosine recombinant mutant SerRS12, pH 7.2, 30°C Saccharomyces cerevisiae
8.5
-
 serine hydroxamate recombinant mutant SerRS11, pH 7.2, 30°C Saccharomyces cerevisiae
18.1
-
 serine hydroxamate recombinant mutant SerRS12, pH 7.2, 30°C Saccharomyces cerevisiae