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Literature summary for 6.1.1.11 extracted from
- Dual mode recognition of two isoacceptor tRNAs by mammalian mitochondrial seryl-tRNA synthetase (2001), J. Biol. Chem., 276, 46770-46778.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expresssion of the mitochondrial isozyme in Escherichia coli BL21(DE3) | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics with diverse mutant variants of tRNASerUGA and tRNASerGCU with the recombinant mitochondrial isozyme, overview | Bos taurus | |
| 0.22 | - |
tRNASerUGA | native wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
| 0.29 | - |
tRNASerUGA | recombinant wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
| 0.35 | - |
tRNASerGCU | recombinant wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
| 0.37 | - |
tRNASerGCU | native wild-type enzyme, pH 8.5, 37°C | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
2 * 57000, recombinant mitochondrial isozyme, SDS-PAGE | Bos taurus |
| 109000 | - |
recombinant mitochondrial isozyme, gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASer | Bos taurus | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | Q9N0F3 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-serine + tRNASer = AMP + diphosphate + L-seryl-tRNASer | tRNA recoginition mechanism, dual recognition mode for isoacceptors, the mitochondrial isozyme specifically recognizes the TPiC loop sequence in each isoacceptor | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASer | - |
Bos taurus | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
| ATP + L-serine + tRNASerGCU | GCU for codon AGY, unusual tRNASer substrate, the mitochondrial isozyme aminoacylates both mitochondrial tRNASer types, the first corresponding to the codon AGY lacking the entire D arm | Bos taurus | AMP + diphosphate + L-seryl-tRNASerGCU | - |
? | |
| ATP + L-serine + tRNASerUGA | UGA for codon UCN, unusual tRNASer substrate, the mitochondrial isozyme aminoacylates both mitochondrial tRNASer types, the second corresponding to the codon UCN with a different cloverleaf structure | Bos taurus | AMP + diphosphate + L-seryl-tRNASerUGA | - |
? | |
| additional information | substrate specificity with diverse mutant variants of tRNASerUGA and tRNASerGCU with the recombinant mitochondrial isozyme, overview | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 57000, recombinant mitochondrial isozyme, SDS-PAGE | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 62 kDa RNA-binding protein | - |
Bos taurus |
| Serine transfer RNA synthetase | - |
Bos taurus |
| Serine translase | - |
Bos taurus |
| Serine--tRNA ligase | - |
Bos taurus |
| SerRS | - |
Bos taurus |
| SerRSmt | - |
Bos taurus |
| SERSEC | - |
Bos taurus |
| Seryl-transfer ribonucleate synthetase | - |
Bos taurus |
| Seryl-transfer ribonucleic acid synthetase | - |
Bos taurus |
| Seryl-transfer RNA synthetase | - |
Bos taurus |
| Seryl-tRNA synthetase | - |
Bos taurus |
| Synthetase, seryl-transfer ribonucleate | - |
Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kcat for diverse mutant variants of tRNASerUGA and tRNASerGCU with the recombinant mitochondrial isozyme, overview | Bos taurus | |
| 0.35 | - |
tRNASerGCU | native wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
| 0.63 | - |
tRNASerUGA | native wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
| 0.64 | - |
tRNASerGCU | recombinant wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
| 0.67 | - |
tRNASerUGA | recombinant wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
| 6.08 | - |
tRNASerUGA | native wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
| 6.08 | - |
tRNASerUGA | recombinant wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
| 6.08 | - |
tRNASerGCU | recombinant wild-type enzyme, pH 8.5, 37°C | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bos taurus | |
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