Activating Compound | Comment | Organism | Structure |
---|---|---|---|
putrescine | enhances activity, optimum concentrations is 0.07 mM | Thermococcus kodakarensis | |
spermidine | enhances activity, optimum concentrations is 0.07 mM | Thermococcus kodakarensis | |
spermine | enhances activity, optimum concentrations is 0.005 mM | Thermococcus kodakarensis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus kodakarensis |
overexpression in Escherichia coli | Pyrococcus sp. |
General Stability | Organism |
---|---|
polyamines stabilize the conformation of the tRNA-AspRS complex | Pyrococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
wild-type and N-terminal 32-residue truncated form (hDRS delta 32) expressed in Escherichia coli | - |
Pyrococcus sp. | - |
overexpressed in Escherichia coli | - |
Pyrococcus sp. KOD1 | - |
overexpressed in Escherichia coli | - |
Thermococcus kodakarensis | Q52428 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus sp. |
- |
Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | - |
Pyrococcus sp. | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Homo sapiens | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Thermococcus kodakarensis | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Pyrococcus sp. KOD1 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
GTP + L-aspartate + tRNAAsp | - |
Pyrococcus sp. | GMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
GTP + L-aspartate + tRNAAsp | - |
Thermococcus kodakarensis | GMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
GTP + L-aspartate + tRNAAsp | - |
Pyrococcus sp. KOD1 | GMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
UTP + L-aspartate + tRNAAsp | - |
Pyrococcus sp. | UMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
UTP + L-aspartate + tRNAAsp | - |
Thermococcus kodakarensis | UMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
UTP + L-aspartate + tRNAAsp | - |
Pyrococcus sp. KOD1 | UMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
ASPA | - |
Thermococcus kodakarensis |
Aspartyl-tRNA synthetase | - |
Thermococcus kodakarensis |
AspRS | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Pyrococcus sp. |
65 | - |
optimum temperature shifts from 65°C to 75°C in the presence of polyamine molecules putrescine, spermine or spermidine | Thermococcus kodakarensis |
75 | - |
in presence of polyamine molecules, such as putrescine, spermine, and spermidine | Pyrococcus sp. |
75 | - |
optimum temperature shifts from 65°C to 75°C in the presence of polyamine molecules putrescine, spermine or spermidine | Thermococcus kodakarensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 75 | 35°C: about 60% of maximal activity, 75°C: about 50% of maximal activity | Thermococcus kodakarensis |
45 | 85 | 45°C: about 80% of maximal activity, 85°C: about 25% of maximal activity | Pyrococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
no denaturation below | Pyrococcus sp. |
85 | - |
the thermal melting temperature seems higher than 85°C in the presence of Mg2+. In the absence of Mg 2+, melting temperature decreases to 85°C | Thermococcus kodakarensis |
90 | - |
half-life is 17 h | Pyrococcus sp. |
90 | - |
half-life: 17 h | Thermococcus kodakarensis |
100 | - |
half-life is 3.5 h | Pyrococcus sp. |
100 | - |
half-life: 3.5 h | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pyrococcus sp. |
7.5 | - |
- |
Thermococcus kodakarensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | pH 5.5: about 60% of maximal activity, pH 9.0: about 30% of maximal activity | Pyrococcus sp. |
5.5 | 8 | pH 5.5: about 60% of maximal activity, pH 8.0: about 65% of maximal activity | Thermococcus kodakarensis |