Activating Compound | Comment | Organism | Structure |
---|---|---|---|
putrescine | can replace Mg2+ in activation with 27% efficiency in aminoacylation | Vigna radiata var. radiata | |
spermidine | can replace Mg2+ in activation with 16% efficiency in aminoacylation | Vigna radiata var. radiata | |
spermine | can replace Mg2+ in activation with 32% efficiency in aminoacylation | Vigna radiata var. radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-aminomalonic acid | inhibits transfer of Asp to tRNA | Vigna radiata var. radiata | |
Br- | 0.7 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
CH3COO- | 2.0 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
Cl- | 2.2 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
ClO3- | 0.1 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
CNS- | 0.1 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
Cs+ | 1.1 M, 50% inhibition of ATP-diphosphate exchange, 0.16 M, 50% inhibition of aminoacylation | Vigna radiata var. radiata | |
erythro-3-hydroxyaspartic acid | inhibits transfer of Asp to tRNA | Vigna radiata var. radiata | |
I- | 0.1 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
IO3- | 0.04 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
K+ | 2.3 M, 50% inhibition of ATP-diphosphate exchange, 0.22 M, 50% inhibition of aminoacylation | Vigna radiata var. radiata | |
Li+ | 0.6 M, 50% inhibition of ATP-diphosphate exchange, 0.11 M, 50% inhibition of aminoacylation | Vigna radiata var. radiata | |
Na+ | 1.4 M, 50% inhibition of ATP-diphosphate exchange, 0.18 M , 50% inhibition of aminoacylation | Vigna radiata var. radiata | |
NH4+ | 0.9 M, 50% inhibition of ATP-diphosphate exchange, 0.14 M, 50% inhibition of aminoacylation | Vigna radiata var. radiata | |
NO3- | 0.7 M, 50% inhibition of ATP-diphosphate exchange | Vigna radiata var. radiata | |
p-chloromercuribenzoate | - |
Vigna radiata var. radiata | |
threo-3-hydroxyaspartic acid | inhibits transfer of Asp to tRNA | Vigna radiata var. radiata | |
threo-3-Methylaspartic acid | inhibits transfer of Asp to tRNA | Vigna radiata var. radiata | |
tRNA | above 0.4 mg/ml | Vigna radiata var. radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
Asp | ATP-diphosphate exchange | Vigna radiata var. radiata | |
8 | - |
2-aminomalonic acid | ATP-diphosphate exchange | Vigna radiata var. radiata | |
24 | - |
threo-3-hydroxyaspartic acid | ATP-diphosphate exchange | Vigna radiata var. radiata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Mg2+, with 12% efficiency in activation of ATP-diphosphate exchange, with 34% efficiency in aminoacylation | Vigna radiata var. radiata | |
Mg2+ | required | Vigna radiata var. radiata | |
Mg2+ | optimal concentration: 12 mM, ATP-diphosphate exchange, 5 mM, aminoacylation | Vigna radiata var. radiata | |
Mg2+ | Km: 6.9 mM , ATP-diphosphate exchange | Vigna radiata var. radiata | |
Mg2+ | Km: 1.1 mM , aminoacylation | Vigna radiata var. radiata | |
Mn2+ | can replace Mg2+, with 35% efficiency in activation of ATP-diphosphate exchange, with 56% efficiency in aminoacylation | Vigna radiata var. radiata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna radiata var. radiata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vigna radiata var. radiata |
Renatured (Comment) | Organism |
---|---|
denaturation by 4 M urea, 30% recovery after dialysis | Vigna radiata var. radiata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.326 | - |
- |
Vigna radiata var. radiata |
Storage Stability | Organism |
---|---|
-20°C, 40% w/v glycerol, SH-group stabilizing reagent, 50% loss of activity after 72 h | Vigna radiata var. radiata |
in a dry form, e.g. as acetone precipitate, stable for 1 month | Vigna radiata var. radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-aminomalonic acid + tRNAAsp | - |
Vigna radiata var. radiata | ? | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Vigna radiata var. radiata | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + threo-3-hydroxyaspartic acid + tRNAAsp | - |
Vigna radiata var. radiata | ? | - |
? | |
additional information | enzyme can also utilize 2-aminomalonic acid and threo-3-hydroxyaspartic acid in ATP-diphosphate exchange | Vigna radiata var. radiata | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life: 7 min without stabilizing reagents, 12 min in presence of 0.004 mM ATP, 23 min in presence of 0.004 mM ATP and 0.1 mM Asp | Vigna radiata var. radiata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
ATP-diphosphate exchange | Vigna radiata var. radiata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.8 | 9.1 | 7.8: sharp loss of activity below, 9.1: plateau of high activity up to pH 9.1 | Vigna radiata var. radiata |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
6000 | - |
Li+ | ATP-diphosphate exchange | Vigna radiata var. radiata |