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Literature summary for 6.1.1.12 extracted from

  • Sauter, C.; Lorber, B.; Cavarelli, J.; Moras, D.; Giege, R.
    The free yeast aspartyl-tRNA synthetase differs from the tRNA(Asp)-complexed enzyme by structural changes in the catalytic site, hinge region, and anticodon-binding domain (2000), J. Mol. Biol., 299, 1313-1324.
    View publication on PubMed
Search for more literature for 6.1.1.12

Cloned(Commentary)

Cloned (Comment) Organism
truncated enzyme lacking 70 amino acid residues in the N-terminus, overexpression in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant truncated enzyme, tri- and tetragonal crystals, X-ry structure determination at 3 and 2.3 A resolution, respectively, molecular replacement, structure analysis and modeling Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsp Saccharomyces cerevisiae
-
 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P04802 dimeric class II aspartyl-tRNA synthetase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant truncated enzyme from Escherichia coli Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsp
-
 Saccharomyces cerevisiae AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp tRNA undergoes large conformational changes upon binding to the enzyme, specific charging of amino acid resdiue on tRNA, accurate recognition by the enzyme is achieved through sequence and structural signalling Saccharomyces cerevisiae AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Synonyms

Synonyms Comment Organism
Aspartic acid translase
-
 Saccharomyces cerevisiae
Aspartyl ribonucleate synthetase
-
 Saccharomyces cerevisiae
aspartyl ribonuleic synthetase
-
 Saccharomyces cerevisiae
Aspartyl-transfer ribonucleic acid synthetase
-
 Saccharomyces cerevisiae
Aspartyl-transfer RNA synthetase
-
 Saccharomyces cerevisiae
Aspartyl-tRNA synthetase
-
 Saccharomyces cerevisiae
AspRS
-
 Saccharomyces cerevisiae