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Literature summary for 6.1.1.14 extracted from

  • Johanson, K.; Hoang, T.; Sheth, M.; Hyman, L.E.
    GRS1, a yeast tRNA synthetase with a role in mRNA 3' end formation (2003), J. Biol. Chem., 278, 35923-35930.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and p552F mutant enzyme in Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
P552F temperature-sensitive mutant grs1-1 shows altered substrate specificities, the mutant strain can be complemented by expression of the wild-type enzyme Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information dissociation constants for complexes of wild-type or mutant enzyme with tRNA and mRNA Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
x * 72000, wild-type enzyme, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glycine + tRNAGly Saccharomyces cerevisiae
-
AMP + diphosphate + glycyl-tRNAGly
-
?
additional information Saccharomyces cerevisiae enzyme plays an important role in transcription termination by affecting 3'-end formation ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-atgged wild-type enzyme and P552F mutant from Escherichia coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycine + tRNAGly
-
Saccharomyces cerevisiae AMP + diphosphate + glycyl-tRNAGly
-
?
ATP + glycine + tRNAGly wild-type and mutant tRNAGly substrates Saccharomyces cerevisiae AMP + diphosphate + glycyl-tRNAGly
-
?
additional information enzyme binds the 3'-ends of mRNA from yeast competing with the cognate tRNAGly substrate Saccharomyces cerevisiae ?
-
?
additional information enzyme plays an important role in transcription termination by affecting 3'-end formation Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
? x * 72000, wild-type enzyme, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Glycine--tRNA ligase
-
Saccharomyces cerevisiae
Glycyl translase
-
Saccharomyces cerevisiae
Glycyl-transfer ribonucleate synthetase
-
Saccharomyces cerevisiae
Glycyl-transfer ribonucleic acid synthetase
-
Saccharomyces cerevisiae
Glycyl-transfer RNA synthetase
-
Saccharomyces cerevisiae
Glycyl-tRNA synthetase
-
Saccharomyces cerevisiae
GlyRS
-
Saccharomyces cerevisiae
Synthetase, glycyl-transfer ribonucleate
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae