Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and p552F mutant enzyme in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
P552F | temperature-sensitive mutant grs1-1 shows altered substrate specificities, the mutant strain can be complemented by expression of the wild-type enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | dissociation constants for complexes of wild-type or mutant enzyme with tRNA and mRNA | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
x * 72000, wild-type enzyme, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + tRNAGly | Saccharomyces cerevisiae | - |
AMP + diphosphate + glycyl-tRNAGly | - |
? | |
additional information | Saccharomyces cerevisiae | enzyme plays an important role in transcription termination by affecting 3'-end formation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-atgged wild-type enzyme and P552F mutant from Escherichia coli | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + tRNAGly | - |
Saccharomyces cerevisiae | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | wild-type and mutant tRNAGly substrates | Saccharomyces cerevisiae | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
additional information | enzyme binds the 3'-ends of mRNA from yeast competing with the cognate tRNAGly substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | enzyme plays an important role in transcription termination by affecting 3'-end formation | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, wild-type enzyme, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Glycine--tRNA ligase | - |
Saccharomyces cerevisiae |
Glycyl translase | - |
Saccharomyces cerevisiae |
Glycyl-transfer ribonucleate synthetase | - |
Saccharomyces cerevisiae |
Glycyl-transfer ribonucleic acid synthetase | - |
Saccharomyces cerevisiae |
Glycyl-transfer RNA synthetase | - |
Saccharomyces cerevisiae |
Glycyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
GlyRS | - |
Saccharomyces cerevisiae |
Synthetase, glycyl-transfer ribonucleate | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |