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Literature summary for 6.1.1.15 extracted from

  • Yaremchuk, A.; Tukalo, M.; Grotli, M.; Cusack, S.
    A succession of substrate induced conformational changes ensures the amino acid specificity of Thermus thermophilus prolyl-tRNA synthetase: comparison with histidyl-tRNA synthetase (2001), J. Mol. Biol., 309, 989-1002.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
native enzyme or enzyme complexed with L-proline, tRNAPro isoacceptors, ATP-Mn2+, prolyl-adenylate intermediate, or prolyl-adenylate analogue Pro-AMS, preparation by soaking of native enzyme crystals in ligand solutions, X-ray diffraction structure determination at 2.85-3.0 A resolution Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-proline + tRNAPro Thermus thermophilus
-
AMP + diphosphate + L-prolyl-tRNAPro
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro induced fit substrate recognition, reaction mechanism, proline binding site structure Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-proline + tRNAPro
-
Thermus thermophilus AMP + diphosphate + L-prolyl-tRNAPro
-
?
ATP + L-proline + tRNAPro binding of L-proline and ATP causes conformational changes in the proline binding loop and motif 2 loop, formation of an activated prolyl-adenylate reaction intermediate, required for the final conformational ordering of a ten residue peptide, the ordered loop, close to the active site Thermus thermophilus AMP + diphosphate + L-prolyl-tRNAPro
-
?

Synonyms

Synonyms Comment Organism
Global RNA synthesis factor
-
Thermus thermophilus
Pro-tRNA synthetase
-
Thermus thermophilus
Proline translase
-
Thermus thermophilus
Proline--tRNA ligase
-
Thermus thermophilus
Prolyl RNA synthetase
-
Thermus thermophilus
Prolyl-transfer ribonucleate synthetase
-
Thermus thermophilus
Prolyl-transfer ribonucleic acid synthetase
-
Thermus thermophilus
Prolyl-transfer RNA synthetase
-
Thermus thermophilus
Prolyl-tRNA synthetase
-
Thermus thermophilus
ProRS
-
Thermus thermophilus
ProRSTT
-
Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Thermus thermophilus