Cloned (Comment) | Organism |
---|---|
gene gltX, expression in Escherichia coli is lethal, but a modulated enzyme, lacking a part of the tRNA acceptor stem binding domain, can be recombinantly expressed in Escherichia coli DH5alpha, overexpression in Bacillus subtilis | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, recombinant enzyme expressed in Bacillus subtilis, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGln | Bacillus subtilis | - |
AMP + diphosphate + L-glutamyl-tRNAGln | - |
? | |
ATP + L-glutamate + tRNAGlu | Bacillus subtilis | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
additional information | Bacillus subtilis | the recombinant wild-type enzyme is toxic for Escherichia coli, probably due to its charging of both tRNAGlu and tRNAGln | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
50fold, recombinant enzyme from overexpression in Bacillus subtilis, to homogeneity | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
purified recombinant enzyme expressed in Bacillus subtilis | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGln | - |
Bacillus subtilis | AMP + diphosphate + L-glutamyl-tRNAGln | - |
? | |
ATP + L-glutamate + tRNAGlu | - |
Bacillus subtilis | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
additional information | the recombinant wild-type enzyme is toxic for Escherichia coli, probably due to its charging of both tRNAGlu and tRNAGln | Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, recombinant enzyme expressed in Bacillus subtilis, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
GluRS | - |
Bacillus subtilis |
Glutamyl-tRNA synthetase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bacillus subtilis |