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Literature summary for 6.1.1.17 extracted from

  • Pelchat, M.; Lacoste, L.; Yang, F.; Lapointe, J.
    Overproduction of the Bacillus subtilis glutamyl-tRNA synthetase in its host and its toxicity to Escherichia coli (1998), Can. J. Microbiol., 44, 378-381.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene gltX, expression in Escherichia coli is lethal, but a modulated enzyme, lacking a part of the tRNA acceptor stem binding domain, can be recombinantly expressed in Escherichia coli DH5alpha, overexpression in Bacillus subtilis Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
x * 55000, recombinant enzyme expressed in Bacillus subtilis, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + tRNAGln Bacillus subtilis
-
AMP + diphosphate + L-glutamyl-tRNAGln
-
?
ATP + L-glutamate + tRNAGlu Bacillus subtilis
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
additional information Bacillus subtilis the recombinant wild-type enzyme is toxic for Escherichia coli, probably due to its charging of both tRNAGlu and tRNAGln ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
50fold, recombinant enzyme from overexpression in Bacillus subtilis, to homogeneity Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
purified recombinant enzyme expressed in Bacillus subtilis Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + tRNAGln
-
Bacillus subtilis AMP + diphosphate + L-glutamyl-tRNAGln
-
?
ATP + L-glutamate + tRNAGlu
-
Bacillus subtilis AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
additional information the recombinant wild-type enzyme is toxic for Escherichia coli, probably due to its charging of both tRNAGlu and tRNAGln Bacillus subtilis ?
-
?

Subunits

Subunits Comment Organism
? x * 55000, recombinant enzyme expressed in Bacillus subtilis, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
GluRS
-
Bacillus subtilis
Glutamyl-tRNA synthetase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Bacillus subtilis