Crystallization (Comment) | Organism |
---|---|
crystallization of the enzyme in different complexes: 1. non-productively complexed with ATP and L-glutamate, 2. with ATP, 3. with tRNAGlu and ATP, 4. with tRNAGlu and the glutamyl-AMP analogue glutamol-AMP, hanging-drop method, 0.008 ml of 5.0 mg/ml protein in 10 mM Na-MOPS, pH 6.5, 5 mM MgCl2, 2.5 mM 2-mercaptoethanol, 1% PEG 6000, 1-2 mM ATP and/or 2 mM glutamate and/or 0.5 mM glutamol-AMP, plus 1 ml reservoir solution containing 10% PEG 6000 at 4 or 20°C, 3 days or more, X-ray diffraction structure determination at 1.8 A resolution, molecular replacement, and analysis | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutamol-AMP | competitive inhibition | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlu | Thermus thermophilus | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | P27000 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-glutamate + tRNAGlu = AMP + diphosphate + L-glutamyl-tRNAGlu | catalytic site structure, substrate binding and reaction mechanism | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlu | - |
Thermus thermophilus | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu | tRNAGlu binding causes conformational changes in the enzyme, glutamine binding mechanism, in presence or absence of tRNA | Thermus thermophilus | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GluRS | - |
Thermus thermophilus |
Glutamyl-tRNA synthetase | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | binding mechanism, in presence or absence of tRNA | Thermus thermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
glutamol-AMP | pH 7.5, 65°C | Thermus thermophilus |