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Literature summary for 6.1.1.19 extracted from

  • Parfait, R.; Grosjean, H.
    Arginyl-transfer ribonucleic-acid synthetase from Bacillus stearothermophilus. Purification, properties and mechanism of action (1972), Eur. J. Biochem., 30, 242-249.
    View publication on PubMed
Search for more literature for 6.1.1.19

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00018
-
 tRNAArg ATP-diphosphate exchange, pH 6.0 Geobacillus stearothermophilus
0.0002
-
 tRNAArg substrate from E. coli, , ATP-diphosphate exchange, pH 6.0 Geobacillus stearothermophilus
0.00031
-
 tRNAArg aminoacylation Geobacillus stearothermophilus
0.0019
-
 Arg aminoacylation Geobacillus stearothermophilus
0.0025
-
 tRNAArg pH 7.4, ATP-diphosphate exchange Geobacillus stearothermophilus
0.0036
-
 tRNAArg substrate from E. coli, , ATP-diphosphate exchange, pH 7.4 Geobacillus stearothermophilus
0.54
-
 ATP aminoacylation Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus NCA 1518
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus

Storage Stability

Storage Stability Organism
-25°C, less than 30% loss of activity after 6 months Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine + tRNAArg Bacillus stearothermophilus tRNAArg, E. coli tRNAArg and yeast tRNAArg (poor) Geobacillus stearothermophilus AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
ATP + L-arginine + tRNAArg nucleotides other than ATP are not effective Geobacillus stearothermophilus AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
ATP + L-arginine + tRNAArg Bacillus stearothermophilus tRNAArg, E. coli tRNAArg and yeast tRNAArg (poor) Geobacillus stearothermophilus NCA 1518 AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
ATP + L-arginine + tRNAArg nucleotides other than ATP are not effective Geobacillus stearothermophilus NCA 1518 AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
additional information arginine-dependent ATP-diphosphate exchange Geobacillus stearothermophilus ?
-
 ?
additional information arginine-dependent ATP-diphosphate exchange Geobacillus stearothermophilus NCA 1518 ?
-
 ?