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Literature summary for 6.1.1.19 extracted from

  • Zhang, Q.S.; Wang, E.D.; Wang, Y.L.
    The role of tryptophan residues in Escherichia coli arginyl-tRNA synthetase (1998), Biochim. Biophys. Acta, 1387, 136-142.
    View publication on PubMed
Search for more literature for 6.1.1.19

Protein Variants

Protein Variants Comment Organism
W162A expression in Escherichia coli, 23% of wild-type activity Escherichia coli
W172A expression in Escherichia coli Escherichia coli
W228A expression in Escherichia coli Escherichia coli
W349A expression in Escherichia coli Escherichia coli
W446A expression in Escherichia coli Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 tRNAArg mutant W162A, pH 7.4, 37ºC Escherichia coli
0.0025
-
 tRNAArg pH 7.4, 37ºC Escherichia coli
0.0073
-
 L-arginine mutant W162A, pH 7.4, 37ºC Escherichia coli
0.012
-
 L-arginine pH 7.4, 37ºC Escherichia coli
0.84
-
 ATP mutant W162A, pH 7.4, 37ºC Escherichia coli
0.9
-
 ATP pH 7.4, 37ºC Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of recombinant enzymes using DEAE-Sepharose and Blue-Sepharose column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 mutant W162A displays 23% of the activity compared to the native enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine + tRNAArg
-
 Escherichia coli AMP + diphosphate + L-arginyl-tRNAArg
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.6
-
 ATP mutant W162A, pH 7.4, 37ºC Escherichia coli
26
-
 ATP pH 7.4, 37ºC Escherichia coli