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Literature summary for 6.1.1.19 extracted from

  • Lazard, M.; Agou, F.; Kerjan, P.; Mirande, M.
    The tRNA-dependent activation of arginine by arginyl-tRNA synthetase requires inter-domain communication (2000), J. Mol. Biol., 302, 991-1004.
    View publication on PubMed
Search for more literature for 6.1.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expression of the native and mutant enzymes in Saccharomyces cerevisiae Cricetulus griseus

Protein Variants

Protein Variants Comment Organism
C599Y expression in Saccharomices cerevisiae, shows reduced activity and stability Cricetulus griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km values for ATP and L-arginine for several mutants and revertants Cricetulus griseus
0.0007
-
 L-arginine ATP-diphosphate exchange reaction, pH 8, 25°C Cricetulus griseus
0.0013
-
 L-arginine aminoacylation reaction, pH 8, 25°C Cricetulus griseus
0.0023
-
 L-arginine ATP-diphosphate exchange reaction, pH 8, 37°C Cricetulus griseus
0.0024
-
 L-arginine aminoacylation reaction, pH 8, 37°C Cricetulus griseus
0.0032
-
 tRNAArg aminoacylation reaction, pH 8, 25°C Cricetulus griseus
0.0044
-
 tRNAArg aminoacylation reaction, pH 8, 37°C Cricetulus griseus
0.19
-
 ATP aminoacylation reaction, pH 8, 25°C Cricetulus griseus
0.24
-
 ATP aminoacylation reaction, pH 8, 37°C Cricetulus griseus

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of the native and mutant enzymes expressed in Saccharomices cerevisiae, using Q-Sepharose and S-Sepharose chromatography Cricetulus griseus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity for different mutants Cricetulus griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine + tRNAArg
-
 Cricetulus griseus AMP + diphosphate + L-arginyl-tRNAArg
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 stable for 6 h Cricetulus griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information values for ATP and L-arginine for several mutants and revertants Cricetulus griseus