Cloned (Comment) | Organism |
---|---|
full-length enzyme and mutant DELTA1-72 tagged with green fluorescence protein, expression in 293 T cells, exclusively in cytosol | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00005 | - |
tRNAArg | 37°C, pH 7.5, arginylation, full-length enzyme | Homo sapiens | |
0.000098 | - |
tRNAArg | 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 | Homo sapiens | |
0.0035 | - |
L-arginine | 37°C, pH 7.5, arginylation, full-length enzyme | Homo sapiens | |
0.0048 | - |
L-arginine | 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 | Homo sapiens | |
0.671 | - |
ATP | 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 | Homo sapiens | |
0.91 | - |
ATP | 37°C, pH 7.5, arginylation, full-length enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | Homo sapiens | the C-terminal extension of the human cytosolic leucyl-tRNA synthetase is indispensable for its interaction with the N-terminal of human cytosolic arginyl-tRNA synthetase in the macromolecular complex | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Homo sapiens | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
ATP + L-arginine + tRNAArg | the C-terminal extension of the human cytosolic leucyl-tRNA synthetase is indispensable for its interaction with the N-terminal of human cytosolic arginyl-tRNA synthetase in the macromolecular complex | Homo sapiens | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
ATP | 37°C, pH 7.5, arginylation, full-length enzyme | Homo sapiens | |
0.32 | - |
tRNAArg | 37°C, pH 7.5, arginylation, full-length enzyme | Homo sapiens | |
0.41 | - |
L-arginine | 37°C, pH 7.5, arginylation, full-length enzyme | Homo sapiens | |
0.77 | - |
ATP | 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 | Homo sapiens | |
0.81 | - |
tRNAArg | 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 | Homo sapiens | |
0.97 | - |
L-arginine | 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 | Homo sapiens |