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Literature summary for 6.1.1.19 extracted from

  • Ling, C.; Yao, Y.N.; Zheng, Y.G.; Wei, H.; Wang, L.; Wu, X.F.; Wang, E.D.
    The C-terminal appended domain of human cytosolic leucyl-tRNA synthetase is indispensable in its interaction with arginyl-tRNA synthetase in the multi-tRNA synthetase complex (2005), J. Biol. Chem., 280, 34755-34763.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
full-length enzyme and mutant DELTA1-72 tagged with green fluorescence protein, expression in 293 T cells, exclusively in cytosol Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00005
-
tRNAArg 37°C, pH 7.5, arginylation, full-length enzyme Homo sapiens
0.000098
-
tRNAArg 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 Homo sapiens
0.0035
-
L-arginine 37°C, pH 7.5, arginylation, full-length enzyme Homo sapiens
0.0048
-
L-arginine 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 Homo sapiens
0.671
-
ATP 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 Homo sapiens
0.91
-
ATP 37°C, pH 7.5, arginylation, full-length enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-arginine + tRNAArg Homo sapiens the C-terminal extension of the human cytosolic leucyl-tRNA synthetase is indispensable for its interaction with the N-terminal of human cytosolic arginyl-tRNA synthetase in the macromolecular complex AMP + diphosphate + L-arginyl-tRNAArg
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine + tRNAArg
-
Homo sapiens AMP + diphosphate + L-arginyl-tRNAArg
-
?
ATP + L-arginine + tRNAArg the C-terminal extension of the human cytosolic leucyl-tRNA synthetase is indispensable for its interaction with the N-terminal of human cytosolic arginyl-tRNA synthetase in the macromolecular complex Homo sapiens AMP + diphosphate + L-arginyl-tRNAArg
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
ATP 37°C, pH 7.5, arginylation, full-length enzyme Homo sapiens
0.32
-
tRNAArg 37°C, pH 7.5, arginylation, full-length enzyme Homo sapiens
0.41
-
L-arginine 37°C, pH 7.5, arginylation, full-length enzyme Homo sapiens
0.77
-
ATP 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 Homo sapiens
0.81
-
tRNAArg 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 Homo sapiens
0.97
-
L-arginine 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 Homo sapiens