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Literature summary for 6.1.1.19 extracted from

  • Yang, F.; Xia, X.; Lei, H.Y.; Wang, E.D.
    Hemin binds to human cytoplasmic arginyl-tRNA synthetase and inhibits its catalytic activity (2010), J. Biol. Chem., 285, 39437-39446.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.19

Cloned(Commentary)

Cloned (Comment) Organism
construction of pMFT7H6-hcArgRS by inserting the full-length hcArgRS gene into the gap between NcoI and BamH1 of vector pMFT7H6. Expression of His-tagged wild-type full-length and truncated mutant enzymes in Escherichia coli strain BL21-CodonPlus (DE3)-RIL Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
hemin hemin is a protoporphyrin containing a ferric iron in the center. ArgRS Cys115 acts as a specific axial ligand of hemin binding that is located in the Add1 domain, but hemin binding to Cys115 is not responsible for the inhibition of enzymatic activity. Hemin inhibits the catalytic activity of full-length and N-terminal 72-amino acid-truncated hcArgRSs by blocking amino acid activation. Hemin induces oligomerization of both the isolated Add1 domain and the wild type enzyme, which might account for the inhibition of catalytic activity. Km values for tRNAArg, arginine, and ATP in the presence of hemin are not altered, but kcat values dramatically decrease compared with those in the absence of hemin, kinetic analysis, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km values for tRNAArg, arginine, and ATP in the presence of hemin are not altered, but kcat values dramatically decrease compared with those in the absence of hemin, kinetic analysis, overview Homo sapiens
0.0065
-
 L-arginine pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin Homo sapiens
0.0078
-
 L-arginine pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin Homo sapiens
0.0081
-
 tRNAArg pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin, Escherichia coli tRNAArg Homo sapiens
0.0096
-
 tRNAArg pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin, Escherichia coli tRNAArg Homo sapiens
0.501
-
 ATP pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin Homo sapiens
0.529
-
 ATP pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-arginine + tRNAArg Homo sapiens
-
 AMP + diphosphate + L-arginyl-tRNAArg
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type full-length and truncated mutant enzymes from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography and gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 comparison of aminoacylation activity of Escherichia coli ArgRS and human truncated DELTANhcArgRS in presence of hemin, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine + tRNAArg
-
 Homo sapiens AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
ATP + L-arginine + tRNAArg substrate is Escherichia coli tRNAArg Homo sapiens AMP + diphosphate + L-arginyl-tRNAArg
-
 ?

Synonyms

Synonyms Comment Organism
Arginyl-tRNA synthetase
-
 Homo sapiens
ArgRS
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
 tRNAArg pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin, Escherichia coli tRNAArg Homo sapiens
3.4
-
 ATP pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin Homo sapiens
3.8
-
 L-arginine pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin Homo sapiens
15.5
-
 ATP pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin Homo sapiens
16.5
-
 tRNAArg pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin, Escherichia coli tRNAArg Homo sapiens
17.9
-
 L-arginine pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function the free form of human cytoplasmic arginyl-tRNA synthetase participates in ubiquitin-dependent protein degradation by offering arginyl-tRNAArg to arginyl-tRNA transferase, ATE1, overview Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6
-
 ATP pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin Homo sapiens
30
-
 ATP pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin Homo sapiens
300
-
 tRNAArg pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin, Escherichia coli tRNAArg Homo sapiens
500
-
 L-arginine pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin Homo sapiens
1700
-
 tRNAArg pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin, Escherichia coli tRNAArg Homo sapiens
2800
-
 L-arginine pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin Homo sapiens