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Literature summary for 6.1.1.19 extracted from
- The influence of identity elements on the aminoacylation of tRNAArg by plant and Escherichia coli arginyl-tRNA synthetases (2012), FEBS J., 279, 3622-3638.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Codon Plus RP cells | Canavalia ensiformis |
| expressed in Escherichia coli BL21 Codon Plus RP cells | Glycine max |
| expressed in Escherichia coli BL21 Codon Plus RP cells | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00029 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Escherichia coli | |
| 0.0014 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Canavalia ensiformis | |
| 0.0017 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Glycine max | |
| 0.0022 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Canavalia ensiformis | |
| 0.0024 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Glycine max | |
| 0.0041 | - |
tRNAArg(ACG) | tRNA from Glycine max. pH and temperature not specified in the publication | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canavalia ensiformis | B2G3G6 | - |
- |
| Escherichia coli | B1XHE4 | - |
- |
| Glycine max | B6ETP1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Source 15Q column chromatography | Glycine max |
| Ni-NTA column chromatography and Source15Q column chromatography | Canavalia ensiformis |
| Ni-NTA column chromatography and Source15Q column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-arginine + tRNAArg(ACG) | tRNA from Escherichia coli | Canavalia ensiformis | AMP + diphosphate + L-arginyl tRNAArg(ACG) | - |
? |  |
| ATP + L-arginine + tRNAArg(ACG) | tRNA from Glycine max | Canavalia ensiformis | AMP + diphosphate + L-arginyl tRNAArg(ACG) | - |
? | |
| ATP + L-arginine + tRNAArg(ACG) | tRNA from Glycine max | Escherichia coli | AMP + diphosphate + L-arginyl-soybean tRNAArg(ACG) | - |
? | |
| ATP + L-arginine + tRNAArg(ACG) | tRNA from Escherichia coli | Glycine max | AMP + diphosphate + L-arginyl-tRNAArg(ACG) | - |
? | |
| ATP + L-arginine + tRNAArg(ACG) | tRNA from Glycine max | Glycine max | AMP + diphosphate + L-arginyl-tRNAArg(ACG) | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Arginyl-tRNA synthetase | - |
Canavalia ensiformis |
| Arginyl-tRNA synthetase | - |
Glycine max |
| Arginyl-tRNA synthetase | - |
Escherichia coli |
| ArgRS | - |
Canavalia ensiformis |
| ArgRS | - |
Glycine max |
| ArgRS | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Escherichia coli | |
| 0.02 | - |
tRNAArg(ACG) | tRNA from Glycine max. pH and temperature not specified in the publication | Escherichia coli | |
| 0.03 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Glycine max | |
| 0.04 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Glycine max | |
| 0.055 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Canavalia ensiformis | |
| 0.105 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Canavalia ensiformis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Canavalia ensiformis | |
| ATP | - |
Glycine max | |
| ATP | - |
Escherichia coli | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.8 | - |
tRNAArg(ACG) | tRNA from Glycine max. pH and temperature not specified in the publication | Escherichia coli | |
| 5.5 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Escherichia coli | |
| 14 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Glycine max | |
| 22.7 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Glycine max | |
| 25.2 | - |
tRNAArg(ACG) | tRNA from Escherichia coli, pH and temperature not specified in the publication | Canavalia ensiformis | |
| 75 | - |
tRNAArg(ACG) | tRNA from Glycine max, pH and temperature not specified in the publication | Canavalia ensiformis |
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