Any feedback?
Literature summary for 6.1.1.2 extracted from
- A dispensable peptide from Acidithiobacillus ferrooxidans tryptophanyl-tRNA synthetase affects tRNA binding (2002), FEBS Lett., 532, 387-390.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence analysis, expression of wild-type enzyme and a mutant enzyme, truncated in the CP2 domain, in Escherichia coli DH5alpha as fusion proteins with glutathione S-transferase | Acidithiobacillus ferrooxidans |
| expression in strain DH5alpha | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00031 | - |
tRNATrp | recombinant wild-type | Acidithiobacillus ferrooxidans |  |
| 0.00062 | - |
tRNATrp | recombinant mutant | Escherichia coli | |
| 0.0015 | - |
L-tryptophan | recombinant wild-type | Acidithiobacillus ferrooxidans | |
| 0.0019 | - |
L-tryptophan | recombinant truncated mutant | Acidithiobacillus ferrooxidans | |
| 0.002 | - |
tRNATrp | recombinant truncated mutant | Acidithiobacillus ferrooxidans | |
| 0.017 | - |
L-tryptophan | recombinant enzyme | Escherichia coli | |
| 0.129 | - |
ATP | recombinant wild-type | Acidithiobacillus ferrooxidans | |
| 0.252 | - |
ATP | recombinant truncated mutant | Acidithiobacillus ferrooxidans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + tRNATrp | Acidithiobacillus ferrooxidans | - |
AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
| ATP + L-tryptophan + tRNATrp | Escherichia coli | - |
AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidithiobacillus ferrooxidans | - |
gene trpS | - |
| Escherichia coli | - |
gene trpS | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + tRNATrp | - |
Acidithiobacillus ferrooxidans | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
| ATP + L-tryptophan + tRNATrp | - |
Escherichia coli | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TrpRS | - |
Acidithiobacillus ferrooxidans |
| TrpRS | - |
Escherichia coli |
| Tryptophanyl-tRNA synthase | - |
Acidithiobacillus ferrooxidans |
| Tryptophanyl-tRNA synthase | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.22 | - |
tRNATrp | recombinant truncated mutant | Acidithiobacillus ferrooxidans | |
| 2.94 | - |
tRNATrp | recombinant truncated mutant | Acidithiobacillus ferrooxidans | |
| 3 | 6 | tRNATrp | recombinant wild-type | Acidithiobacillus ferrooxidans | |
| 3.17 | - |
tRNATrp | recombinant wild-type | Acidithiobacillus ferrooxidans | |
| 13.6 | - |
L-tryptophan | recombinant truncated mutant | Acidithiobacillus ferrooxidans | |
| 13.9 | - |
tRNATrp | recombinant mutant | Escherichia coli | |
| 24.5 | - |
L-tryptophan | recombinant wild-type | Acidithiobacillus ferrooxidans | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Acidithiobacillus ferrooxidans | |
| ATP | - |
Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
