Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.2 extracted from

  • Xu, F.; Jia, J.; Jin, Y.; Wang, D.T.
    High-level expression and single-step purification of human tryptophanyl-tRNA synthetase (2001), Protein Expr. Purif., 23, 296-300.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene trpS, overexpression in Escherichia coli strain BL21(DE3) derivative as a His-tagged protein Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
tRNATrp pH 7.5, 30°C, recombinant enzyme Homo sapiens
0.0074
-
L-tryptophan pH 7.5, 30°C, recombinant enzyme Homo sapiens
0.088
-
ATP pH 7.5, 30°C, recombinant enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tryptophan + tRNATrp Homo sapiens
-
AMP + diphosphate + L-tryptophan-tRNATrp
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
large sclae, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) derivative, 2.5fold, to homogeneity Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0018
-
purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tryptophan + tRNATrp
-
Homo sapiens AMP + diphosphate + L-tryptophan-tRNATrp
-
?

Synonyms

Synonyms Comment Organism
TrpRS
-
Homo sapiens
Tryptophanyl-tRNA synthase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
tRNATrp pH 7.5, 30°C, recombinant enzyme Homo sapiens
1.1
-
ATP pH 7.5, 30°C, recombinant enzyme Homo sapiens
1.1
-
L-tryptophan pH 7.5, 30°C, recombinant enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens