Literature summary for 6.1.1.2 extracted from

Yang, X.L.; Otero, F.J.; Ewalt, K.L.; Liu, J.; Swairjo, M.A.; Koehrer, C.; RajBhandary, U.L.; Skene, R.J.; McRee, D.E.; Schimmel, P.
Two conformations of a crystalline human tRNA synthetase-tRNA complex: implications for protein synthesis (2006), EMBO J., 25, 2919-2929.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified protein in complex with uncharged tRNA and aminoacylated tRNA in a 1:1 mixture, sitting drop vapour diffusion method, 0.002 ml of protein in 10 mM HEPES, pH 7.5, 20 mM KCl, 0.02% NaN3, and 2 mM 2-mercaptoethanol, addition of 230 mM of TrpRS, 250 mM of tRNATrp, 5 mM tryptophan and 10 mM AMP-PNP, mixed with 0.002 ml reservoir solution containing 2 M ammonium sulfate and 0.1 M HEPES pH 6.9, 4°C, X-ray diffraction structure determination and analysis at 2.9 A resolution, SAD method using a selenium-labeled crystal	Homo sapiens

Crystallization (Comment)

Organism

purified protein in complex with uncharged tRNA and aminoacylated tRNA in a 1:1 mixture, sitting drop vapour diffusion method, 0.002 ml of protein in 10 mM HEPES, pH 7.5, 20 mM KCl, 0.02% NaN3, and 2 mM 2-mercaptoethanol, addition of 230 mM of TrpRS, 250 mM of tRNATrp, 5 mM tryptophan and 10 mM AMP-PNP, mixed with 0.002 ml reservoir solution containing 2 M ammonium sulfate and 0.1 M HEPES pH 6.9, 4°C, X-ray diffraction structure determination and analysis at 2.9 A resolution, SAD method using a selenium-labeled crystal

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	preparation of alpha17 deletion mini-TrpRS, DELTAD382Q389, by site-directed mutagensis, overview	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-tryptophan + tRNATrp	Homo sapiens	-	AMP + diphosphate + L-tryptophanyl-tRNATrp	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P23381	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + L-tryptophan + tRNATrp	-	Homo sapiens	AMP + diphosphate + L-tryptophanyl-tRNATrp	-	?
ATP + L-tryptophan + tRNATrp	tRNA substrate from Bos taurus, tryptophan binding pocket structure, overview, two distinct tRNA conformations: uncharged tRNA is bound across the dimer, with anticodon and acceptor stem interacting with separate subunits, in this cross-dimer tRNA complex, the class I enzyme has a class II-like tRNA binding mode, the aminoacylated tRNA is bound only by the anticodon, the acceptor stem being free and having space to interact precisely with EF-1a, suggesting that the product of aminoacylation can be directly handed off to EF-1alpha for the next step of protein synthesis, recognition mechanisms, overview	Homo sapiens	AMP + diphosphate + L-tryptophanyl-tRNATrp	-	?
additional information	the enzyme interacts directly with elongation factor 1alpha, which carries charged tRNA to the ribosome	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
More	secondary structure determination and analysis, sequence comparisons, overview	Homo sapiens

Synonyms

Synonyms	Comment	Organism
TrpRS	-	Homo sapiens
Tryptophanyl-tRNA synthetase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at, aminoacylation	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at, aminoacylation	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens