Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00005 | - |
Archeoglobus fulgidus tRNATrp(A73C) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.00005 | - |
Archeoglobus fulgidus tRNATrp(A73U) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.00006 | - |
Archeoglobus fulgidus tRNATrp(A73, wild-type) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.00006 | - |
Archeoglobus fulgidus tRNATrp(A73G) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.00009 | - |
Bacillus subtilis tRNATrp(A73, wild-type) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.0001 | - |
Bacillus subtilis tRNATrp(A73G) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.00012 | - |
Bacillus subtilis tRNATrp(A73C) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.00012 | - |
Bacillus subtilis tRNATrp(A73U) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.00017 | - |
bovine tRNATrp(G73U) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.00018 | - |
bovine tRNATrp(G73, wild-type) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.00021 | - |
bovine tRNATrp(G73A) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.00022 | - |
bovine tRNATrp(G73C) | pH 7.0, 65°C | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O28579 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73, wild-type) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73, wild-type) | - |
? | |
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73C) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73C) | - |
? | |
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73G) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73G) | - |
? | |
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73U) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73U) | - |
? | |
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73, wild-type) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73, wild-type) | - |
? | |
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73C) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73C) | - |
? | |
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73G) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73G) | - |
? | |
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73U) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73U) | - |
? | |
ATP + L-tryptophan + bovine tRNATrp(G73, wild-type) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73, wild-type) | - |
? | |
ATP + L-tryptophan + bovine tRNATrp(G73A) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73A) | - |
? | |
ATP + L-tryptophan + bovine tRNATrp(G73C) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73C) | - |
? | |
ATP + L-tryptophan + bovine tRNATrp(G73U) | preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) | Archaeoglobus fulgidus | AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73U) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TrpRS | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Archaeoglobus fulgidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
bovine tRNATrp(G73, wild-type) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.04 | - |
bovine tRNATrp(G73U) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.04 | - |
Bacillus subtilis tRNATrp(A73G) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.05 | - |
Bacillus subtilis tRNATrp(A73U) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.06 | - |
bovine tRNATrp(G73C) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.06 | - |
Bacillus subtilis tRNATrp(A73C) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.07 | - |
Archeoglobus fulgidus tRNATrp(A73G) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.08 | - |
bovine tRNATrp(G73A) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.09 | - |
Archeoglobus fulgidus tRNATrp(A73U) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.11 | - |
Archeoglobus fulgidus tRNATrp(A73C) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.14 | - |
Bacillus subtilis tRNATrp(A73, wild-type) | pH 7.0, 65°C | Archaeoglobus fulgidus | |
0.53 | - |
Archeoglobus fulgidus tRNATrp(A73, wild-type) | pH 7.0, 65°C | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Archaeoglobus fulgidus |