Literature summary for 6.1.1.2 extracted from

Khan, S.; Garg, A.; Sharma, A.; Camacho, N.; Picchioni, D.; Saint-Leger, A.; Ribas de Pouplana, L.; Yogavel, M.; Sharma, A.
An appended domain results in an unusual architecture for malaria parasite tryptophanyl-tRNA synthetase (2013), PLoS ONE, 8, e66224.

Search for more literature for 6.1.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli B834 cells	Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with L-tryptophan, hanging drop vapor diffusion method, using 0.03 M Na-NO3+ (0.03 M ammonium sulfate, 0.3 M Na2HPO4), 0.1 M bicin pH 8.5, 12.5% (w/v) 2-methyl-2,4-pentanediol, 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, at 20°C	Plasmodium falciparum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Plasmodium falciparum	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
73000	-	2 * 73000, SDS-PAGE	Plasmodium falciparum
140000	-	gel filtration	Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-tryptophan + tRNATrp	Plasmodium falciparum	-	AMP + diphosphate + L-tryptophyl-tRNATrp	-	?

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	Q8IDW3	-	-

Purification (Commentary)

Purification (Comment)	Organism
heparin column chromatography and Superdex 200 gel filtration	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-tryptophan + tRNATrp	-	Plasmodium falciparum	AMP + diphosphate + L-tryptophyl-tRNATrp	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 73000, SDS-PAGE	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
Tryptophanyl-tRNA synthetase	-	Plasmodium falciparum
WRS	-	Plasmodium falciparum

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Plasmodium falciparum

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Release 2023.1 (January 2023)