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Literature summary for 6.1.1.2 extracted from
- The plant aminoacyl-tRNA synthetases. Purification and characterization of valyl-tRNA, tryptophanyl-tRNA and seryl-tRNA synthetases from yellow lupin seeds (1975), Eur. J. Biochem., 52, 301-310.
General Stability
| General Stability | Organism |
|---|---|
| requires intact -SH groups for their activity | Lupinus luteus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dATP | - |
Lupinus luteus |  |
| NEM | - |
Lupinus luteus | |
| p-hydroxymercuribenzoate | - |
Lupinus luteus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
tRNATrp | - |
Lupinus luteus | |
| 0.005 | - |
Trp | - |
Lupinus luteus | |
| 0.03 | - |
ATP | - |
Lupinus luteus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | optimal concentration: 50 mM | Lupinus luteus | |
| Mg2+ | Km: 1.5 mM | Lupinus luteus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
possibly 4 * 37000, SDS-PAGE | Lupinus luteus |
| 200000 | - |
gel filtration | Lupinus luteus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lupinus luteus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lupinus luteus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Lupinus luteus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.45 | - |
- |
Lupinus luteus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% v/v glycerol, 5 mM potassium phosphate buffer, pH 6.8, 1 mM 2-mercaptoethanol | Lupinus luteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + tRNATrp | - |
Lupinus luteus | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
| additional information | not: dATP | Lupinus luteus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | possibly 4 * 37000, SDS-PAGE | Lupinus luteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | in cacodylate buffer | Lupinus luteus |
| 8 | - |
in phosphate buffer | Lupinus luteus |
| 8.5 | - |
in Hepes buffer | Lupinus luteus |
| 8.5 | 9.2 | in Tris buffer | Lupinus luteus |
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Release 2023.1 (January 2023)
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