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Literature summary for 6.1.1.20 extracted from

  • Bobkova, E.V.; Stepanov, V.G.; Lavrik, O.I.
    A comparative study of the relationship between thermostability and function of phenylalanyl-tRNA synthetase from Escherichia coli and Thermus thermophilus (1992), FEBS Lett., 302, 54-56.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe
-
Thermus thermophilus AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
?
ATP + L-phenylalanine + tRNAPhe
-
Escherichia coli AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43
-
ATP-diphosphate exchange Escherichia coli
85
-
ATP-diphosphate exchange Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44
-
about 50% loss of activity after 50 min Escherichia coli
90
-
about 30% loss of activity after 80 min Thermus thermophilus
95
-
about 45% loss of activity after 20 min Thermus thermophilus