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Literature summary for 6.1.1.20 extracted from

  • Rauhut, R.; Gabius, H.J.; Kuhn, W.; Cramer, F.
    Phenylalanyl-tRNA synthetase from the archaebacterium Methanosarcina barkeri (1984), J. Biol. Chem., 259, 6340-6345.
    View publication on PubMed
Search for more literature for 6.1.1.20

Inhibitors

Inhibitors Comment Organism Structure
3'-Methoxy-ATP
-
 Methanosarcina barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 2-Chloroadenosine 5'-triphosphate aminoacylation Methanosarcina barkeri
0.06
-
 ATP aminoacylation Methanosarcina barkeri
0.81
-
 N6-methyladenosine 5'-triphosphate aminoacylation Methanosarcina barkeri
0.82
-
 3'-Deoxyadenosine 5'-triphosphate aminoacylation Methanosarcina barkeri
1.54
-
 2-deoxyadenosine 5'-triphosphate aminoacylation Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 2 * 63000 (alpha) + 2 * 70000 (beta), SDS-PAGE Methanosarcina barkeri
70000
-
 2 * 63000 (alpha) + 2 * 70000 (beta), SDS-PAGE Methanosarcina barkeri
270000
-
 gel filtration Methanosarcina barkeri

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina barkeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15
-
-
 Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine 5'-triphosphate + L-phenylalanine + tRNAPhe
-
 Methanosarcina barkeri 2'-deoxyadenosine 5'-monophosphate + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
2-chloroadenosine 5'-triphosphate + phenylalanine + tRNAPhe
-
 Methanosarcina barkeri 2-chloroadenosine 5'-monophosphate + diphosphate + phenylalanyl-tRNAPhe
-
 ?
3'-deoxyadenosine 5'-triphosphate + L-phenylalanine + tRNAPhe
-
 Methanosarcina barkeri 3'-deoxyadenosine 5'-monophosphate + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe heterologous aminoacylation of tRNA with high selectivity for archaebacterial tRNA Methanosarcina barkeri AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
N6-methyladenosine 5'-triphosphate + L-phenylalanine + tRNAPhe
-
 Methanosarcina barkeri N6-methyladenosine 5'-monophosphate + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 63000 (alpha) + 2 * 70000 (beta), SDS-PAGE Methanosarcina barkeri