Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.20 extracted from

  • Kodama, K.; Fukuzawa, S.; Sakamoto, K.; Nakayama, H.; Kigawa, T.; Yabuki, T.; Matsuda, N.; Shirouzu, M.; Takio, K.; Tachibana, K.; Yokoyama, S.
    A new protein engineering approach combining chemistry and biology, part I; site-specific incorporation of 4-iodo-L-phenylalanine in vitro by using misacylated suppressor tRNAPhe (2006), Chembiochem, 7, 1577-1581.
    View publication on PubMed
Search for more literature for 6.1.1.20

Activating Compound

Activating Compound Comment Organism Structure
DTT activates Escherichia coli

Application

Application Comment Organism
biotechnology misacylation of suppressor tRNAPhe CUA by the PheRS mutant A294G with 4-iodo-L-phenylalanine might be useful in application in protein engineering since an aryl iodide tag on proteins can be used for site-specific functionalization of proteins, used for cell-free protein synthesis as a stoichiometric reagent, overview Escherichia coli

Protein Variants

Protein Variants Comment Organism
A294G thermosensitive active site mutant, the suppressor tRNAPhe CUA is misacylated with 4-iodo-L-phenylalanine by the mutant at a high magnesium-ion concentration of 70 mM Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe Escherichia coli
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe Escherichia coli NP37
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 carrying the thermosensitive point mutation A294G in the active site
-
Escherichia coli NP37
-
 carrying the thermosensitive point mutation A294G in the active site
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-iodo-L-phenylalanine + amber tRNAPheCUA suppressor tRNAPhe CUA is misacylated with 4-iodo-L-phenylalanine by the mutant at a high magnesium-ion concentration by PheRS mutant A294G Escherichia coli AMP + diphosphate + 4-iodo-L-phenylalanyl-amber tRNAPheCUA
-
 ?
ATP + 4-iodo-L-phenylalanine + amber tRNAPheCUA suppressor tRNAPhe CUA is misacylated with 4-iodo-L-phenylalanine by the mutant at a high magnesium-ion concentration by PheRS mutant A294G Escherichia coli NP37 AMP + diphosphate + 4-iodo-L-phenylalanyl-amber tRNAPheCUA
-
 ?
ATP + L-phenylalanine + tRNAPhe
-
 Escherichia coli AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe
-
 Escherichia coli NP37 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Synonyms

Synonyms Comment Organism
Phenylalanyl-tRNA synthetase
-
 Escherichia coli
PheRS
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.1 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli