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Literature summary for 6.1.1.20 extracted from

  • Reynolds, N.M.; Ling, J.; Roy, H.; Banerjee, R.; Repasky, S.E.; Hamel, P.; Ibba, M.
    Cell-specific differences in the requirements for translation quality control (2010), Proc. Natl. Acad. Sci. USA, 107, 4063-4068.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.20

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli BL21-RIL/pET16b produces His6-tagged mtPheRS encoded by the MSF1 gene, the ctPheRS alpha and beta subunits, encoded by the FRS1 and FRS2 genes, respectively, are expressed in tandem from pQE31-FRS-sc in Escherichia coli BL21-RIL cells Saccharomyces cerevisiae
Escherichia coli pET21c+ encoding human mtPheRS, FARS2, produces mature His6-tagged wild-type PheRS Homo sapiens

Protein Variants

Protein Variants Comment Organism
A294G EcPheRS mutant Escherichia coli
A333G mtPheRS mutant Saccharomyces cerevisiae
G458A ctPheRS mutant Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 L-phenylalanine wild-type EcPheRS Escherichia coli
0.003
-
 L-phenylalanine wild-type ctPheRS Saccharomyces cerevisiae
0.0045
-
 L-phenylalanine mutant A294G EcPheRS Escherichia coli
0.005
-
 L-phenylalanine wild-type mtPheRS Saccharomyces cerevisiae
0.017
-
 L-phenylalanine mutant A333G mtPheRS Saccharomyces cerevisiae
0.233
-
 L-phenylalanine mutant G458A ctPheRS Saccharomyces cerevisiae
0.32
-
 L-tyrosine mutant A294G EcPheRS Escherichia coli
0.637
-
 L-tyrosine wild-type ctPheRS Saccharomyces cerevisiae
0.66
-
 L-tyrosine mutant A333G mtPheRS Saccharomyces cerevisiae
1.155
-
 L-tyrosine wild-type mtPheRS Saccharomyces cerevisiae
2.2
-
 L-tyrosine wild-type EcPheRS Escherichia coli
3
-
 L-tyrosine mutant G458A ctPheRS Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm ctPheRS Saccharomyces cerevisiae 5737
-
mitochondrion
-
 Homo sapiens 5739
-
mitochondrion mtPheRS Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe Escherichia coli
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe Homo sapiens
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe Saccharomyces cerevisiae
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His6-tagged proteins are purified on nickel-nitrilotriacetic acid-agarose Homo sapiens
His6-tagged proteins are purified on nickel-nitrilotriacetic acid-agarose Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe
-
 Escherichia coli AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe
-
 Homo sapiens AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe
-
 Saccharomyces cerevisiae AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-tyrosine + tRNAPhe
-
 Escherichia coli AMP + diphosphate + L-tyrosyl-tRNAPhe
-
 ?
ATP + L-tyrosine + tRNAPhe
-
 Homo sapiens AMP + diphosphate + L-tyrosyl-tRNAPhe
-
 ?
ATP + L-tyrosine + tRNAPhe
-
 Saccharomyces cerevisiae AMP + diphosphate + L-tyrosyl-tRNAPhe
-
 ?

Subunits

Subunits Comment Organism
heterotetramer ctPheRS, alpha and beta-subunits, the alpha-subunits contain the actice site, the beta-subunits harbor the editing site Saccharomyces cerevisiae
heterotetramer EcPheRS, alpha and beta-subunits, the alpha-subunits contain the actice site, the beta-subunits harbor the editing site Escherichia coli
monomer
-
 Homo sapiens
monomer mtPheRS, alpha-subunit monomer, catalytic domain Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
ctPheRS cytoplasmic type Saccharomyces cerevisiae
EcPheRS
-
 Escherichia coli
mtPheRS mitochondrial type Homo sapiens
mtPheRS mitochondrial type Saccharomyces cerevisiae
Phenylalanyl-tRNA synthetase
-
 Escherichia coli
Phenylalanyl-tRNA synthetase
-
 Homo sapiens
Phenylalanyl-tRNA synthetase
-
 Saccharomyces cerevisiae
PheRS
-
 Escherichia coli
PheRS
-
 Homo sapiens
PheRS
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 L-tyrosine mutant G458S ctPheRS Saccharomyces cerevisiae
5
-
 L-tyrosine wild-type mtPheRS Saccharomyces cerevisiae
12.5
-
 L-tyrosine mutant A333G mtPheRS Saccharomyces cerevisiae
35
-
 L-tyrosine wild-type EcPheRS Escherichia coli
140
-
 L-phenylalanine mutant A333G mtPheRS Saccharomyces cerevisiae
180
-
 L-phenylalanine wild-type mtPheRS Saccharomyces cerevisiae
184
-
 L-tyrosine wild-type ctPheRS Saccharomyces cerevisiae
185
-
 L-phenylalanine mutant A294G EcPheRS Escherichia coli
185
-
 L-tyrosine mutant A294G EcPheRS Escherichia coli
199
-
 L-phenylalanine wild-type EcPheRS Escherichia coli
464
-
 L-phenylalanine mutant G45A8 ctPheRS Saccharomyces cerevisiae
603
-
 L-phenylalanine wild-type ctPheRS Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli
ATP
-
 Homo sapiens
ATP
-
 Saccharomyces cerevisiae