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Literature summary for 6.1.1.20 extracted from
- Cloning of Bombyx mori phenylalanyl-tRNA synthetase and the generation of its mutant with relaxed amino acid specificity (2010), J. Insect Biotechnol. Sericol., 79, 53-65.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| two genes encoding subunits alpha and beta, DNA and amino acid sequence determination and analysis, expression as His-tagged proteins in Escherichia coli strain M15 | Bombyx mori |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A450G | mutation in subunit alpha, tRNAPhe substrate specificity and flexibilty in charging pPhe variants compared to the wild-type enzyme | Bombyx mori |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Bombyx mori |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
1 * 54000, alpha-subunit, + 1 * 62000, beta-subunit, SDS-PAGE | Bombyx mori |
| 62000 | - |
1 * 54000, alpha-subunit, + 1 * 62000, beta-subunit, SDS-PAGE | Bombyx mori |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | Bombyx mori | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged proteins from Escherichia coli strain M15 by nickel affinity chromatography, complex formation of the two subunits during purification | Bombyx mori |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| silk gland | - |
Bombyx mori | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | - |
Bombyx mori | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 54000, alpha-subunit, + 1 * 62000, beta-subunit, SDS-PAGE | Bombyx mori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Phenylalanyl-tRNA synthetase | - |
Bombyx mori |
| PheRS | - |
Bombyx mori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bombyx mori |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | no activity at 10°C and 50°C | Bombyx mori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bombyx mori |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | no activity below pH 6.0 and above 8.5, higher activity range is pH 6.5-8.0 | Bombyx mori |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bombyx mori | |
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