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Literature summary for 6.1.1.20 extracted from

  • Moor, N.; Klipcan, L.; Safro, M.G.
    Bacterial and eukaryotic phenylalanyl-tRNA synthetases catalyze misaminoacylation of tRNAPhe with 3,4-dihydroxy-L-phenylalanine (2011), Chem. Biol., 18, 1221-1229.
    View publication on PubMed
Search for more literature for 6.1.1.20

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with 3,4-dihydroxy-L-phenylalanine Thermus thermophilus
in complex with 3,4-dihydroxy-L-phenylalanine Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
DL-m-tyrosine
-
 Homo sapiens
L-tyrosine
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 L-phenylalanine in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Thermus thermophilus
0.0026
-
 L-phenylalanine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.0032
-
 L-phenylalanine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.012
-
 DL-m-tyrosine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.13
-
 DL-m-tyrosine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.38
-
 3,4-dihydroxy-L-phenylalanine in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Thermus thermophilus
0.6
-
 3,4-dihydroxy-L-phenylalanine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.65
-
 3,4-dihydroxy-L-phenylalanine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
1.9
-
 L-tyrosine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-
mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe Thermus thermophilus
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe Homo sapiens
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens O95363
-
-
Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3,4-dihydroxy-L-phenylalanine + tRNAPhe
-
 Thermus thermophilus AMP + diphosphate + 3,4-dihydroxy-L-phenylalanyl-tRNAPhe
-
 ?
ATP + 3,4-dihydroxy-L-phenylalanine + tRNAPhe 0.13% activity compared to L-phenylalanine Homo sapiens AMP + diphosphate + 3,4-dihydroxy-L-phenylalanyl-tRNAPhe
-
 ?
ATP + 3,4-dihydroxy-L-phenylalanine + tRNAPhe 0.33% activity compared to L-phenylalanine Homo sapiens AMP + diphosphate + 3,4-dihydroxy-L-phenylalanyl-tRNAPhe
-
 ?
ATP + DL-m-tyrosine + tRNAPhe 1.9% activity compared to L-phenylalanine Homo sapiens AMP + diphosphate + DL-m-tyrosyl-tRNAPhe
-
 ?
ATP + DL-m-tyrosine + tRNAPhe 22% activity compared to L-phenylalanine Homo sapiens AMP + diphosphate + DL-m-tyrosyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe
-
 Thermus thermophilus AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe
-
 Homo sapiens AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-phenylalanine + tRNAPhe 100% activity Homo sapiens AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
ATP + L-tyrosine + tRNAPhe 0.089% activity compared to L-phenylalanine Homo sapiens AMP + diphosphate + L-tyrosyl-tRNAPhe
-
 ?

Synonyms

Synonyms Comment Organism
Phenylalanyl-tRNA synthetase
-
 Thermus thermophilus
Phenylalanyl-tRNA synthetase
-
 Homo sapiens
PheRS
-
 Thermus thermophilus
PheRS
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.033
-
 L-tyrosine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.052
-
 DL-m-tyrosine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.057
-
 3,4-dihydroxy-L-phenylalanine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.075
-
 L-phenylalanine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.16
-
 3,4-dihydroxy-L-phenylalanine in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Thermus thermophilus
0.23
-
 3,4-dihydroxy-L-phenylalanine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.65
-
 DL-m-tyrosine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.87
-
 L-phenylalanine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
1.12
-
 L-phenylalanine in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Thermus thermophilus
ATP
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0136
-
 DL-m-tyrosine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.15
-
 DL-m-tyrosine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
3.4
-
 L-tyrosine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.017
-
 L-tyrosine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.095
-
 3,4-dihydroxy-L-phenylalanine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.37
-
 3,4-dihydroxy-L-phenylalanine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
0.43
-
 3,4-dihydroxy-L-phenylalanine in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Thermus thermophilus
4.3
-
 DL-m-tyrosine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
5
-
 DL-m-tyrosine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
28.3
-
 L-phenylalanine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
270
-
 L-phenylalanine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
650
-
 L-phenylalanine in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Thermus thermophilus