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Literature summary for 6.1.1.22 extracted from

  • Berthet-Colominas, C.; Seignovert, L.; Hartlein, M.; Grotli, M.; Cusack, S.; Leberman, R.
    The crystal structure of asparaginyl-tRNA synthetase from Thermus thermophilus and its complexes with ATP and asparaginyl-adenylate: the mechanism of discrimination between asparagine and aspartic acid (1998), EMBO J., 17, 2947-2960.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.22

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant enzyme, native or complexed withATP and asparaginyl-adenylate, 3 different crystal forms, X-ray diffraction structure determination at 2.6 A resolution and analysis Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-asparagine + tRNAAsn Thermus thermophilus
-
 AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus P54263 purified recombinant enzyme expressed in Escherichia coli
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-asparagine + tRNAAsn = AMP + diphosphate + L-asparaginyl-tRNAAsn mechanism of substrate specificity, structure of substrate binding sites Thermus thermophilus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-asparagine + tRNAAsn
-
 Thermus thermophilus AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?
ATP + L-asparagine + tRNAAsn substrate specificity Thermus thermophilus AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?

Subunits

Subunits Comment Organism
dimer class II synthetase, crystal structure determination Thermus thermophilus

Synonyms

Synonyms Comment Organism
AsnRS
-
 Thermus thermophilus
Asparagine translase
-
 Thermus thermophilus
Asparagine--tRNA ligase
-
 Thermus thermophilus
Asparaginyl transfer ribonucleic acid synthetase
-
 Thermus thermophilus
Asparaginyl transfer RNA synthetase
-
 Thermus thermophilus
Asparaginyl-transfer ribonucleate synthetase
-
 Thermus thermophilus
Asparaginyl-tRNA synthetase
-
 Thermus thermophilus
Asparagyl-transfer RNA synthetase
-
 Thermus thermophilus
Potentially protective 63 kDa antigen
-
 Thermus thermophilus
Synthetase, asparaginyl-transfer ribonucleate
-
 Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Thermus thermophilus